从景观噬菌体展示文库中筛选出的经典猪瘟病毒特异性配体。
Specific ligands for classical swine fever virus screened from landscape phage display library.
作者信息
Yin Long, Luo Yuzi, Liang Bo, Wang Fei, Du Min, Petrenko Valery A, Qiu Hua-Ji, Liu Aihua
机构信息
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China; The Laboratory for Biosensing, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China.
出版信息
Antiviral Res. 2014 Sep;109:68-71. doi: 10.1016/j.antiviral.2014.06.012. Epub 2014 Jun 28.
Classical swine fever (CSF) is a devastating infectious disease caused by classical swine fever virus (CSFV). The screening of CSFV-specific ligands is of great significance for diagnosis and treatment of CSF. Affinity selection from random peptide libraries is an efficient approach to discover ligands with high stability and specificity. Here, we screened phage ligands for the CSFV E2 protein from f8/8 landscape phage display library by biopanning and obtained four phage clones specific for the E2 protein of CSFV. Viral blocking assays indicated that the phage clone displaying the octapeptide sequence DRATSSNA remarkably inhibited the CSFV replication in PK-15 cells at a titer of 10(10) transduction units, as evidenced by significantly decreased viral RNA copies and viral titers. The phage-displayed E2-binding peptides have the potential to be developed as antivirals for CSF.
经典猪瘟（CSF）是由经典猪瘟病毒（CSFV）引起的一种毁灭性传染病。筛选CSFV特异性配体对于CSF的诊断和治疗具有重要意义。从随机肽库中进行亲和选择是发现具有高稳定性和特异性配体的有效方法。在此，我们通过生物淘选从f8/8景观噬菌体展示文库中筛选针对CSFV E2蛋白的噬菌体配体，并获得了四个对CSFV E2蛋白具有特异性的噬菌体克隆。病毒阻断试验表明，展示八肽序列DRATSSNA的噬菌体克隆在效价为10(10)转导单位时显著抑制PK-15细胞中CSFV的复制，病毒RNA拷贝数和病毒滴度显著降低证明了这一点。噬菌体展示的E2结合肽有潜力被开发为CSF的抗病毒药物。
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