牛尸体病变部位及内脏中布鲁氏菌属DNA的实时荧光定量PCR检测
Real-time PCR detection of Brucella spp. DNA in lesions and viscera of bovine carcasses.
作者信息
Sola Marília Cristina, da Veiga Jardim Eurione A G, de Freitas Marcius Ribeiro, de Mesquita Albenones José
机构信息
Centro de Pesquisa em Alimentos, Universidade Federal de Goiás, Goiânia, GO, Brazil.
Centro de Pesquisa em Alimentos, Universidade Federal de Goiás, Goiânia, GO, Brazil.
出版信息
J Microbiol Methods. 2014 Sep;104:87-91. doi: 10.1016/j.mimet.2014.06.015. Epub 2014 Jun 28.
This study reports a real-time PCR assay for the detection of Brucella spp. associated with the FTA® Elute method in lesions observed during sanitary inspections in beef slaughter. Of the total 276 samples, 78 (28.3%) tested positive and 198 (71.7%) negative for Brucella spp. The real-time PCR technique associated with the FTA® Elute method proved to be an important tool for the diagnosis, judgment about and disposal of carcasses and viscera of slaughtered animals.
本研究报告了一种用于检测布鲁氏菌属的实时荧光定量聚合酶链反应（PCR）检测方法，该方法与FTA®洗脱法相关，用于牛肉屠宰卫生检查中观察到的病变。在总共276个样本中，78个（28.3%）布鲁氏菌属检测呈阳性，198个（71.7%）呈阴性。与FTA®洗脱法相关的实时荧光定量PCR技术被证明是诊断、判断和处理屠宰动物胴体及内脏的重要工具。
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