Fitzgerald L R, Glick S D, Schneider A S
Department of Pharmacology and Toxicology, Albany Medical College, NY 12208.
Brain Res. 1989 Mar 6;481(2):373-7. doi: 10.1016/0006-8993(89)90817-2.
Rats lesioned unilaterally with 6-hydroxydopamine, as an animal model of Parkinson's disease, were tested for D-amphetamine-induced rotation. Buffer or isolated bovine adrenal chromaffin cells were implanted into the lesioned striatum. Rats were retested for rotation 1 and 4 weeks postimplantation. Those with surviving implants rotated significantly less than controls. HPLC assay of striata showed that implants contained elevated norepinephrine and epinephrine, but not dopamine. The results show that the bovine chromaffin cell implants can function as a restorative treatment for nigrostriatal damage, that neonatal implants work better than adult implants, and that such restoration occurs independent of dopamine levels.
用6-羟基多巴胺单侧损伤的大鼠作为帕金森病的动物模型，接受了右旋苯丙胺诱导的旋转测试。将缓冲液或分离的牛肾上腺嗜铬细胞植入损伤的纹状体。在植入后1周和4周对大鼠进行旋转复测。植入物存活的大鼠旋转明显少于对照组。对纹状体的高效液相色谱分析表明，植入物中去甲肾上腺素和肾上腺素含量升高，但多巴胺含量未升高。结果表明，牛嗜铬细胞植入物可作为黑质纹状体损伤的一种修复性治疗方法，新生动物植入物比成年动物植入物效果更好，且这种修复与多巴胺水平无关。
