Trattner Sigal, Kashdan Eugene, Feigin Micha, Sochen Nir
J Opt Soc Am A Opt Image Sci Vis. 2014 May 1;31(5):968-80. doi: 10.1364/JOSAA.31.000968.
The differential-interference-contrast (DIC) microscope is of widespread use in life sciences as it enables noninvasive visualization of transparent objects. The goal of this work is to model the image formation process of thick three-dimensional objects in DIC microscopy. The model is based on the principles of electromagnetic wave propagation and scattering. It simulates light propagation through the components of the DIC microscope to the image plane using a combined geometrical and physical optics approach and replicates the DIC image of the illuminated object. The model is evaluated by comparing simulated images of three-dimensional spherical objects with the recorded images of polystyrene microspheres. Our computer simulations confirm that the model captures the major DIC image characteristics of the simulated object, and it is sensitive to the defocusing effects.
微分干涉对比（DIC）显微镜在生命科学中被广泛使用，因为它能够对透明物体进行非侵入式成像。这项工作的目标是对DIC显微镜中厚三维物体的成像过程进行建模。该模型基于电磁波传播和散射原理。它使用几何光学和物理光学相结合的方法模拟光通过DIC显微镜的各个组件传播到图像平面的过程，并复制被照亮物体的DIC图像。通过将三维球形物体的模拟图像与聚苯乙烯微球的记录图像进行比较来评估该模型。我们的计算机模拟证实，该模型捕捉到了模拟物体的主要DIC图像特征，并且对散焦效应敏感。
