Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China;Center for Information Biology and
Laboratory of Developmental Genetics, Shandong Agricultural University, Tai'an, Shandong 271018, China;
Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9497-502. doi: 10.1073/pnas.1407145111. Epub 2014 Jun 16.
During Drosophila eye development, differentiation initiates in the posterior region of the eye disk and progresses anteriorly as a wave marked by the morphogenetic furrow (MF), which demarcates the boundary between anterior undifferentiated cells and posterior differentiated photoreceptors. However, the mechanism underlying the regulation of gene expression immediately before the onset of differentiation remains unclear. Here, we show that Apontic (Apt), which is an evolutionarily conserved transcription factor, is expressed in the differentiating cells posterior to the MF. Moreover, it directly induces the expression of cyclin E and is also required for the G1-to-S phase transition, which is known to be essential for the initiation of cell differentiation at the MF. These observations identify a pathway crucial for eye development, governed by a mechanism in which Cyclin E promotes the G1-to-S phase transition when regulated by Apt.
在果蝇眼睛发育过程中，分化从眼盘的后区域开始，并以前部波状形态向前推进，该波状形态由形态发生沟（MF）标记，MF 界定了前部未分化细胞和后部分化光感受器之间的边界。然而，在分化开始之前，调节基因表达的机制仍不清楚。在这里，我们表明，Apt（一种进化上保守的转录因子）在 MF 后分化的细胞中表达。此外，它直接诱导细胞周期蛋白 E 的表达，并且还需要 G1 到 S 期的转变，已知该转变对于 MF 处细胞分化的开始至关重要。这些观察结果确定了一个对眼睛发育至关重要的途径，该途径受一种机制调控，当受 Apt 调控时，细胞周期蛋白 E 促进 G1 到 S 期的转变。
