Safdar M, Junejo Y, Arman Kaifee, Abasıyanık M F
Department of Medical Biology and Genetics, University of Gaziantep, Gaziantep, Turkey.
Department of Medical Biology and Genetics, University of Gaziantep, Gaziantep, Turkey; National Center of Excellence in Analytical Chemistry, University of Sindh Jamshoro, Jamshoro 76080, Pakistan.
Meat Sci. 2014 Oct;98(2):296-300. doi: 10.1016/j.meatsci.2014.06.006. Epub 2014 Jun 13.
A tetraplex PCR assay was developed for a rapid and reliable identification of horse, soybean, poultry, and pork species in sausages simultaneously. The method merges the use of horse (Equus caballus), soybean (Glycine max), poultry (Gallus gallus), and pork (Sus scrofa) specific primers that amplify small fragments (horse; 85bp, soybean; 100bp, poultry; 183bp and pork; 212bp) of the mitochondrial cyt b, lectin, 12S rRNA and ATPase subunit 6 genes respectively. Good quality DNA was isolated from reference sausage to optimize the assay. Tetraplex analysis of the reference sausage samples showed that the detection limit of the assay was 0.01% for each species. Taken together, all data indicated that this tetraplex PCR assay was a simple, rapid, sensitive, specific, and cost-effective detection method for horse, soybean, poultry, and pork species in commercial sausages.
开发了一种四重PCR检测方法，用于同时快速、可靠地鉴定香肠中的马、大豆、家禽和猪肉种类。该方法合并使用了马（马属）、大豆（大豆）、家禽（家鸡）和猪肉（野猪）的特异性引物，这些引物分别扩增线粒体细胞色素b、凝集素、12S rRNA和ATPase亚基6基因的小片段（马；85bp，大豆；100bp，家禽；183bp和猪肉；212bp）。从参考香肠中分离出高质量的DNA以优化检测。对参考香肠样品的四重分析表明，该检测方法对每种物种的检测限为0.01%。综上所述，所有数据表明，这种四重PCR检测方法是一种用于商业香肠中马、大豆、家禽和猪肉种类的简单、快速、灵敏、特异且经济高效的检测方法。
