Hibi E, Osano E, Fujii Y, Moriyama T
Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
FEMS Microbiol Lett. 1989 Jan 15;48(2):121-4. doi: 10.1111/j.1574-6968.1989.tb03284.x.
Chondroitin 6-sulfate depolymerizing activity was examined in the culture supernatant of Streptococcus intermedius ATCC 27335. 2-Acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose was split from the substrate. The enzyme(s) was not active upon chondroitin 4-sulfate or dermatan sulfate, which indicated that the enzyme responsible for the depolymerization is chondroitinase C.
对中间链球菌ATCC 27335培养上清液中的硫酸软骨素6-硫酸酯解聚活性进行了检测。从底物中裂解出2-乙酰氨基-2-脱氧-3-O-（β-D-葡萄糖-4-烯吡喃糖醛酸）-6-O-磺基-D-半乳糖。该酶对硫酸软骨素4-硫酸酯或硫酸皮肤素无活性，这表明负责解聚的酶是软骨素酶C。
