锁核酸（LNA）：用于诊断和治疗的对RNA的高亲和力靶向作用。
Locked nucleic acid (LNA): High affinity targeting of RNA for diagnostics and therapeutics.
作者信息
Kauppinen Sakari, Vester Birte, Wengel Jesper
机构信息
Wilhelm Johannsen Centre for Functional Genome Research, Institute of Medical Biochemistry and Genetics, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark.
Nucleic Acid Center, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark.
出版信息
Drug Discov Today Technol. 2005 Autumn;2(3):287-90. doi: 10.1016/j.ddtec.2005.08.012.
Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. This conformational restriction results in unprecedented hybridization affinity towards complementary single stranded RNA and thus, makes LNA uniquely suited for mimicking RNA structures and sequence specific targeting of RNA in vitro or in vivo. The focus of this paper is on LNA-antisense, LNA-modified siRNA (siLNA), and detection and analysis of microRNAs by LNA-modified oligonucleotide probes.:
锁核酸（LNA）是一种核酸类似物，包含一个或多个带有双环呋喃糖单元的LNA核苷酸单体，该双环呋喃糖单元被锁定在模拟RNA的糖构象中。这种构象限制导致其对互补单链RNA具有前所未有的杂交亲和力，因此，使LNA特别适合于在体外或体内模拟RNA结构以及对RNA进行序列特异性靶向。本文的重点是LNA反义核酸、LNA修饰的小干扰RNA（siLNA），以及通过LNA修饰的寡核苷酸探针检测和分析微小RNA。
Zotero 插件