Chen Xiao, Liu Min, Xiao Jiamei, Yang Weidi, Peng Zaiqing
a Guangxi Key Lab for Mangrove Conservation and Utilization , Guangxi Mangrove Research Center, Guangxi Academy of Sciences , Beihai , P.R. China and.
b College of Ocean and Earth Sciences, Xiamen University , Xiamen , Fujian , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1090-1. doi: 10.3109/19401736.2014.930836. Epub 2014 Jul 1.
The complete mitochondrial genome of Carcharhinus macloti was determined in this study. It is 16,701 bp in length and contains 37 genes with the typical gene order and transcriptional orientation in vertebrates. A total of 29 bp overlaps and 29 bp short intergenic spaces located in 22 gene junctions. The overall base composition is 31.6% A, 26.2% C, 13.0% G and 29.2% T. Two start codons (ATG and GTG) and three stop codons (AGG, TAG and TAA/T) were found in 13 protein-coding genes. The length of 22 tRNA genes ranged from 66 bp (tRNA-Ser2) to 75 bp (tRNA-Leu1). The tRNA-Ser2 (GCU) lacks the dihydrouridine arm by a simple loop and can not be folded into the typical cloverleaf structure. The control region is 1066 bp in length with high A+T content (68.2%).
本研究测定了大齿真鲨的完整线粒体基因组。其长度为16,701 bp，包含37个基因，具有脊椎动物典型的基因顺序和转录方向。共有29 bp的重叠区和29 bp的短基因间隔区位于22个基因连接处。总体碱基组成为31.6%A、26.2%C、13.0%G和29.2%T。在13个蛋白质编码基因中发现了两个起始密码子（ATG和GTG）和三个终止密码子（AGG、TAG和TAA/T）。22个tRNA基因的长度范围为66 bp（tRNA-Ser2）至75 bp（tRNA-Leu1）。tRNA-Ser2（GCU）通过一个简单环缺失二氢尿嘧啶臂，无法折叠成典型的三叶草结构。控制区长度为1066 bp，A+T含量较高（68.2%）。
