虫霉目属的免疫学分离

Immunological separation of Entomophthorales genera.

作者信息

Toriello C, Zerón E, Latgé J P, Mier T

出版信息

J Invertebr Pathol. 1989 May;53(3):358-60. doi: 10.1016/0022-2011(89)90100-6.

DOI:10.1016/0022-2011(89)90100-6

Abstract

Exoantigens from Erynia neoaphidis, Conidiobolus major, C. thromboides, C. obscurus, Zoophthora radicans, and Basidiobolus ranarum were obtained from culture filtrates of fungal material grown in a yeast extract, peptone dialysate, dextrose medium and were tested against specific hyperimmune antisera prepared from E. neoaphidis, C. major, C. thromboides, and B. ranarum by the immunodiffusion technique. Specific precipitins were observed for E. neoaphidis and B. ranarum, while cross-reactions were detected among C. major, C. thromboides, and C. obscurus. The results suggest that genera of Entomophthorales can be easily separated by this simple immunological procedure.

摘要

从新蚜虫疠霉、大耳霉、血栓霉、暗耳霉、根虫瘟霉和蛙粪霉中提取的外抗原取自于在酵母提取物、蛋白胨透析液、葡萄糖培养基中生长的真菌材料的培养滤液，并通过免疫扩散技术针对由新蚜虫疠霉、大耳霉、血栓霉和蛙粪霉制备的特异性超免疫抗血清进行了检测。观察到新蚜虫疠霉和蛙粪霉出现特异性沉淀素，而在大耳霉、血栓霉和暗耳霉之间检测到交叉反应。结果表明，通过这种简单的免疫学方法可以轻松区分虫霉目各属。