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在不同pH值下提取的大豆油体的常量营养素和微量营养素。

Macronutrients and micronutrients of soybean oil bodies extracted at different pH.

作者信息

Chen Yeming, Cao Yanyun, Zhao Luping, Kong Xiangzhen, Hua Yufei

机构信息

State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan Univ, 1800 Lihu Avenue, Wuxi, Jiangsu Province 214122, PR, China.

出版信息

J Food Sci. 2014 Jul;79(7):C1285-91. doi: 10.1111/1750-3841.12516. Epub 2014 Jul 1.

Abstract

UNLABELLED

In this study, the macronutrients and micronutrients of pH 6.8, 8.0, 9.5, and 11.0 extracted soybean oil bodies (OBs) were examined, revealing that soybean OBs might be used as a natural carrier for bioactive components (unsaturated fatty acids, phospholipid, tocopherol, and phytosterol). pH 6.8 extracted OBs (dry basis) contained 85.88% neutral lipid, 8.18% protein, and 5.85% polar lipid (mainly phospholipid) by gravimetric analysis. The percentage of neutral lipid was increased, while those of protein and polar lipid were decreased with increasing pH. Tocopherol (about 75 mg/100 g neutral lipid of OBs) was not affected, while phytosterol was decreased (136 to 110 mg/100 g neutral lipid of OBs) with increasing pH. The detectable total monosaccaride (galactosamine, glucosamine, and glucose) content of extracted OBs was low and also decreased (35.80 to 6.13 mg/100 g neutral lipid of OBs) with increasing pH. The protein of extracted OBs had higher percentage of essential amino acids than soybean protein isolate with tryptophan and methionine as limited amino acids. The fatty acid composition of extracted OBs was rich in linoleic acid (about 59%), oleic acid (about 20%), and α-linolenic acid (about 7%).

PRACTICAL APPLICATION

Oil bodies (OBs) from soybean and other plant seeds are greatly examined owing to their potential utilizations in food ingredients. The determination of its macronutrients and micronutrients would be very meaningful for its efficient utilization in the future.

摘要

未标注

在本研究中,对pH值为6.8、8.0、9.5和11.0的提取大豆油体(OBs)的常量营养素和微量营养素进行了检测,结果表明大豆油体可用作生物活性成分(不饱和脂肪酸、磷脂、生育酚和植物甾醇)的天然载体。通过重量分析,pH值为6.8时提取的OBs(干基)含有85.88%的中性脂质、8.18%的蛋白质和5.85%的极性脂质(主要是磷脂)。随着pH值的升高,中性脂质的百分比增加,而蛋白质和极性脂质的百分比降低。生育酚(约75毫克/100克OBs中性脂质)不受影响,而植物甾醇随着pH值的升高而降低(从136毫克/100克OBs中性脂质降至110毫克/100克OBs中性脂质)。提取的OBs中可检测到的总单糖(半乳糖胺、葡萄糖胺和葡萄糖)含量较低,并且也随着pH值的升高而降低(从35.80毫克/100克OBs中性脂质降至6.13毫克/100克OBs中性脂质)。提取的OBs的蛋白质中必需氨基酸的百分比高于大豆分离蛋白,其中色氨酸和蛋氨酸为限制氨基酸。提取的OBs的脂肪酸组成富含亚油酸(约59%)、油酸(约20%)和α-亚麻酸(约7%)。

实际应用

大豆和其他植物种子的油体(OBs)因其在食品成分中的潜在用途而受到广泛研究。测定其常量营养素和微量营养素对于其未来的高效利用具有重要意义。

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