在仿生胶原蛋白膜上培养的内皮细胞的生物物理化学特性。
Biophysiochemical properties of endothelial cells cultured on bio-inspired collagen films.
作者信息
Seo Eunseok, Seo Kyung Won, Gil Jung-Eun, Ha Young-Ran, Yeom Eunseop, Lee Seungchul, Lee Sang Joon
机构信息
Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk 790-784, Korea.
出版信息
BMC Biotechnol. 2014 Jul 2;14:61. doi: 10.1186/1472-6750-14-61.
BACKGROUND
In this study, we investigated the effect of the extracellular matrix on endothelial dysfunction by careful observation of human umbilical vein endothelial cells (HUVECs) cultured on denatured collagen film.
RESULTS
HUVECs on denatured collagen film showed relatively high surface roughness compared with normal HUVECs. The expression levels of MMP-1, MMP-2 and CD146 increased in the ECs on denatured collagen film. In addition, we examined the accumulation of fluorescent beads on HUVEC layers subjected to circulatory flow. The number of accumulated fluorescent beads increased on the disorganized HUVEC layers.
CONCLUSIONS
The proposed in vitro study using bio-inspired collagen films could potentially be used in the size- and ligand-based design of drugs to treat endothelial dysfunction caused by circulatory vascular diseases.
背景
在本研究中，我们通过仔细观察在变性胶原膜上培养的人脐静脉内皮细胞（HUVECs），研究了细胞外基质对内皮功能障碍的影响。
结果
与正常HUVECs相比，变性胶原膜上的HUVECs表现出相对较高的表面粗糙度。变性胶原膜上的内皮细胞中MMP-1、MMP-2和CD146的表达水平升高。此外，我们检测了循环流动条件下HUVEC层上荧光珠的积累情况。在紊乱的HUVEC层上积累的荧光珠数量增加。
结论
所提出的使用仿生胶原膜的体外研究可能潜在地用于基于尺寸和配体的药物设计，以治疗循环血管疾病引起的内皮功能障碍。
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