狭窄通道周围血流和血小板黏附的微流控测量：瓦片尺寸对相关图中血小板黏附检测的影响。
Microfluidic measurement for blood flow and platelet adhesion around a stenotic channel: Effects of tile size on the detection of platelet adhesion in a correlation map.
作者信息
Jung Sung Yong, Yeom Eunseop
机构信息
Department of Mechanical Engineering, Chosun University, Gwangju, South Korea.
School of Mechanical Engineering, Pusan National University, Busan 46241, South Korea.
出版信息
Biomicrofluidics. 2017 Apr 25;11(2):024119. doi: 10.1063/1.4982605. eCollection 2017 Mar.
Platelet aggregation affects the surrounding blood flow and usually occurs where a blood vessel is narrowed as a result of atherosclerosis. The relationship between blood flow and platelet aggregation is not yet fully understood. This study proposes a microfluidic method to measure the velocity and platelet aggregation simultaneously by combining the micro-particle image velocimetry technique and a correlation mapping method. The blood flow and platelet adhesion procedure in a stenotic micro-channel with 90% severity were observed for a relatively long period of 4 min. In order to investigate the effect of tile size on the detection of platelet adhesion, 2D correlation coefficients were evaluated with binary images obtained by manual labeling and the correlation mapping method with different sizes of the square tile ranging from 3 to 50 pixels. The maximum 2D correlation coefficient occurred with the optimum tile size of 5 × 5 pixels. Since the blood flow and platelet aggregation are mutually influenced by each other, blood flow and platelet adhesion were continuously varied. When there was no platelet adhesion (t = 0 min), typical blood flow is observed. The blood flow passes through the whole channel smoothly, and jet-like flow occurs in the post-stenosis region. However, the flow pattern changes when platelet adhesion starts at the stenosis apex and after the stenosis. These adhesions induce narrow high velocity regions to become wider over a range of area from upstream to downstream of the stenosis. Separated jet-like flows with two high velocity regions are also created. The changes in flow patterns may alter the patterns of platelet adhesion. As the area of the plate adhesion increases, the platelets plug the micro-channel and there is only a small amount of blood flow, finally. The microfluidic method could provide new insights for better understanding of the interactions between platelet aggregation and blood flow in various physiological conditions.
血小板聚集会影响周围的血流，通常发生在因动脉粥样硬化导致血管狭窄的部位。血流与血小板聚集之间的关系尚未完全明确。本研究提出了一种微流控方法，通过结合微粒图像测速技术和相关映射方法来同时测量速度和血小板聚集。在严重程度为90%的狭窄微通道中，对血流和血小板黏附过程进行了长达4分钟的较长时间观察。为了研究方块大小对血小板黏附检测的影响，使用手动标记获得的二值图像以及相关映射方法，对大小从3到50像素不等的不同方形方块评估二维相关系数。最大二维相关系数出现在5×5像素的最佳方块大小处。由于血流和血小板聚集相互影响，血流和血小板黏附不断变化。当没有血小板黏附时（t = 0分钟），观察到典型的血流情况。血流平稳地通过整个通道，在狭窄后区域出现喷射状血流。然而，当血小板在狭窄顶端及狭窄后开始黏附时，流动模式会发生变化。这些黏附会使狭窄上游到下游范围内的狭窄高速区域变宽。还会产生具有两个高速区域的分离喷射状血流。流动模式的变化可能会改变血小板黏附模式。随着血小板黏附面积增加，血小板会堵塞微通道，最终只剩下少量血流。这种微流控方法可为更好地理解不同生理条件下血小板聚集与血流之间的相互作用提供新的见解。
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