用于长期干细胞生长的三维氧化锆混合支架的双光子聚合
Two-photon polymerization of 3-D zirconium oxide hybrid scaffolds for long-term stem cell growth.
作者信息
Skoog Shelby A, Nguyen Alexander K, Kumar Girish, Zheng Jiwen, Goering Peter L, Koroleva Anastasia, Chichkov Boris N, Narayan Roger J
机构信息
Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, North Carolina 27965 and Office of Science and Engineering Laboratories, Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, Maryland 20993.
Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, North Carolina 27965 and Department of Nanotechnology, Laser Zentrum Hannover e. V., Hannover D-30419, Germany.
出版信息
Biointerphases. 2014 Jun;9(2):029014. doi: 10.1116/1.4873688.
Two-photon polymerization is a technique that involves simultaneous absorption of two photons from a femtosecond laser for selective polymerization of a photosensitive material. In this study, two-photon polymerization was used for layer-by-layer fabrication of 3-D scaffolds composed of an inorganic-organic zirconium oxide hybrid material. Four types of scaffold microarchitectures were created, which exhibit layers of parallel line features at various orientations as well as pores between the line features. Long-term cell culture studies involving human bone marrow stromal cells were conducted using these 3-D scaffolds. Cellular adhesion and proliferation were demonstrated on all of the scaffold types; tissuelike structure was shown to span the pores. This study indicates that two-photon polymerization may be used to create microstructured scaffolds out of an inorganic-organic zirconium oxide hybrid material for use in 3-D tissue culture systems.
双光子聚合是一种涉及同时吸收飞秒激光的两个光子以实现光敏材料选择性聚合的技术。在本研究中，双光子聚合用于逐层制造由无机-有机氧化锆杂化材料组成的三维支架。创建了四种类型的支架微结构，它们在不同方向上呈现平行线特征层以及线特征之间的孔隙。使用这些三维支架进行了涉及人骨髓基质细胞的长期细胞培养研究。在所有类型的支架上均证实了细胞黏附和增殖；组织样结构跨越孔隙。本研究表明，双光子聚合可用于由无机-有机氧化锆杂化材料制造微结构支架，以用于三维组织培养系统。
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