Konttinen Y T, Jungell P, Bergroth V, Hampf G, Kemppinen P, Malmström M
Fourth Department of Medicine, Helsinki University Central Hospital and Research Laboratory, Department of Oral Surgery, Finland.
J Clin Lab Immunol. 1989 Jan;28(1):33-7.
Peripheral blood T cell function in five oral lichen planus (OLP) patients and five healthy controls was assessed using different activation parameters. Staining with monoclonal antibodies against interleukin-2 receptor and MHC locus II coded Ia antigen, 3H-thymidine incorporation and gamma-interferon secretion were determined in phytohaemagglutinin (PHA) stimulated peripheral blood mononuclear cell cultures at days 0, 1, 3 and 5. The peripheral blood T cell subsets and spontaneous MHC locus II antigen expression were similar in OLP patients and in controls whereas the spontaneous lymphocyte proliferation was lower in OLP patients than in controls (p less than 0.01). This may reflect a slight in vivo preactivation and its effect on lymphocyte recirculation. The PHA-induced expression of IL-2 receptor and T cell proliferation were similar in both groups whereas gamma-interferon secretion and MHC locus II antigen expression were low in OLP patients compared with controls (p less than 0.01). The results suggest a defect in OLP T cell activation disclosed by in vitro PHA stimulation and localized between IL-2 receptor ligand binding and gamma-interferon secretion.
采用不同的激活参数评估了5例口腔扁平苔藓（OLP）患者和5例健康对照者的外周血T细胞功能。在第0、1、3和5天，对用植物血凝素（PHA）刺激的外周血单个核细胞培养物进行抗白细胞介素-2受体和MHC II类基因座编码的Ia抗原的单克隆抗体染色、3H-胸腺嘧啶核苷掺入及γ-干扰素分泌的测定。OLP患者和对照者的外周血T细胞亚群及自发MHC II类抗原表达相似，而OLP患者的自发淋巴细胞增殖低于对照者（p<0.01）。这可能反映了体内轻微的预激活及其对淋巴细胞再循环的影响。两组中PHA诱导的IL-2受体表达和T细胞增殖相似，而与对照者相比，OLP患者的γ-干扰素分泌和MHC II类抗原表达较低（p<0.01）。结果提示，体外PHA刺激揭示了OLP患者T细胞激活存在缺陷，且该缺陷位于IL-2受体配体结合和γ-干扰素分泌之间。
