Park Hyo Seok, Shin Dong Sun, Cho Dai Hai, Jung Yong Wook, Park Jin Seo
Department of Anatomy, Keimyung University School of Medicine, 1095 Dalgubeol-daero, Daegu 704-701, Republic of Korea.
Department of Anatomy, Ajou University School of Medicine, Suwon 443-749, Republic of Korea.
Ann Anat. 2014 Sep;196(5):352-9. doi: 10.1016/j.aanat.2014.05.036. Epub 2014 Jun 9.
The objective of this research was to produce high-quality sectioned images of a whole dog which can be used to create sectional anatomy atlases and three-dimensional (3D) models. A year old female beagle was sacrificed by potassium chloride injection and frozen. The frozen dog was then serially ground using a cryomacrotome. Sectioned surfaces were photographed using a digital camera to create 3555 sectioned images of whole dog body (intervals, 0.2 mm; pixel size, 0.1 mm; 48 bit color). In a sectioned image, structures of dimension greater than 0.1mm could be identified in detail. Photoshop was used to make segmented images of 16 structures. Sectioned and segmented images were stored in browsing software to allow easy access. Segmented images were reconstructed to make surface models of 16 structures using Mimics software and stored in portable document format (PDF) using Adobe 3D Reviewer software. In this research, state-of-art sectioned images and surface models were produced for the dog. The authors hope that the sectioned images produced will become a useful source of software for basic and clinical veterinary medicine, and therefore, are distributing the sectioned images and surface models through browsing software and PDF file available free of charge.
本研究的目的是制作一只完整犬的高质量切片图像，可用于创建断层解剖图谱和三维（3D）模型。一只一岁的雌性比格犬通过注射氯化钾处死并冷冻。然后使用冷冻切片机对冷冻的犬进行连续研磨。使用数码相机拍摄切片表面，以创建3555张完整犬体的切片图像（间隔0.2毫米；像素大小0.1毫米；48位颜色）。在一张切片图像中，可以详细识别尺寸大于0.1毫米的结构。使用Photoshop制作16种结构的分割图像。将切片图像和分割图像存储在浏览软件中以便于访问。使用Mimics软件对分割图像进行重建，以制作16种结构的表面模型，并使用Adobe 3D Reviewer软件以便携式文档格式（PDF）存储。在本研究中，为犬制作了最先进的切片图像和表面模型。作者希望所制作的切片图像将成为基础和临床兽医学软件的有用来源，因此，正在通过免费提供的浏览软件和PDF文件分发切片图像和表面模型。
