测量钙离子指示剂的速率常数。
Measuring the rate constants of Ca²⁺ indicators.
作者信息
Faas Guido C, Mody Istvan
机构信息
Department of Neurology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095;
Department of Neurology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095; Department of Physiology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095.
出版信息
Cold Spring Harb Protoc. 2014 Jul 1;2014(7):763-7. doi: 10.1101/pdb.prot073288.
Fluorescent Ca(2+) indicators are widely used to measure the concentration of free Ca(2+) (Ca(2+)) in biological processes. To determine the exact kinetics of changes in Ca(2+) and the processes underlying these changes (e.g., Ca(2+) binding to Ca(2+)-binding proteins), it is necessary to know the exact binding properties of the indicator used. Here, we describe how to determine the binding rate constants (k(on) and k(off)) of Ca(2+) indicators.
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