Ghigo E, Imperiale E, Mazza E, Goffi S, Procopio M, Müller E E, Camanni F
Department of Biomedicine, University of Turin, Italy.
Neuroendocrinology. 1989 Feb;49(2):134-7. doi: 10.1159/000125104.
It has been shown that enhanced cholinergic tone induced by pyridostigmine (PD) increases both basal and GHRH-stimulated GH levels in both adults and children. In this study the effects of PD (60 mg orally) on GH secretion were studied both in the morning (from 8.00 to 12.00) and in the night (from 23.00 to 3.00) in 7 short children previously shown as having a normal spontaneous nocturnal GH secretion. In the morning, PD induced a GH increase higher than saline (peak, mean +/- SEM: 17.4 +/- 3.4 vs. 5.5 +/- 3.0 ng/ml, p less than 0.02; area under curve (AUC): 360.8 +/- 71.4 vs. 109.4 +/- 44.7 ng/ml/h, p less than 0.01). In the night, no difference was observed between GH secretion after PD (peak: 16.7 +/- 2.4 ng/ml; AUC: 468.2 +/- 95.5 ng/ml/h) and saline (peak: 16.0 +/- 2.7 ng/ml; AUC: 409.1 +/- 97.7 ng/ml/h). Spontaneous GH secretion was higher during the night than in the morning (p less than 0.02) whereas nocturnal GH secretion overlapped with that in the morning after PD. The ability of PD to increase GH secretion during the morning but not GH hypersecretion occurring at night implies that the cholinergic tone in the central nervous system areas controlling GH secretion is already maximally stimulated at night. Since, reportedly, the cholinergic system negatively modulates somatostatin secretion, presence of a physiologically reduced somatostatinergic tone may be envisaged at night.
已表明，吡啶斯的明（PD）诱导的胆碱能张力增强会使成人和儿童的基础生长激素（GH）水平以及生长激素释放激素（GHRH）刺激的GH水平均升高。在本研究中，对7名先前显示夜间GH自发性分泌正常的矮小儿童，在上午（8点至12点）和夜间（23点至3点）研究了口服60 mg PD对GH分泌的影响。上午，PD诱导的GH升高高于生理盐水（峰值，均值±标准误：17.4±3.4 vs. 5.5±3.0 ng/ml，p<0.02；曲线下面积（AUC）：360.8±71.4 vs. 109.4±44.7 ng/ml/h，p<0.01）。夜间，PD后GH分泌（峰值：16.7±2.4 ng/ml；AUC：468.2±95.5 ng/ml/h）与生理盐水后（峰值：16.0±2.7 ng/ml；AUC：409.1±97.7 ng/ml/h）未观察到差异。夜间的自发性GH分泌高于上午（p<0.02），而夜间GH分泌与PD后上午的GH分泌重叠。PD在上午增加GH分泌但在夜间不增加GH分泌过多的能力表明，控制GH分泌的中枢神经系统区域的胆碱能张力在夜间已受到最大程度刺激。据报道，胆碱能系统对生长抑素分泌起负调节作用，因此可以设想夜间生理上生长抑素能张力降低。
