Engelberth Rovena Clara G J, Silva Kayo Diogenes de A, Azevedo Carolina V de M, Gavioli Elaine Cristina, dos Santos Jose Ronaldo, Soares Joacil Germano, Nascimento Junior Expedito S, Cavalcante Judney C, Costa Miriam Stela M O, Cavalcante Jeferson S
Laboratory of Neurochemical Studies, Physiology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Laboratory of Chronobiology, Physiology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Biomed Res Int. 2014;2014:243825. doi: 10.1155/2014/243825. Epub 2014 Jun 2.
The suprachiasmatic nuclei (SCN) are pointed to as the mammals central circadian pacemaker. Aged animals show internal time disruption possibly caused by morphological and neurochemical changes in SCN components. Some studies reported changes of neuronal cells and neuroglia in the SCN of rats and nonhuman primates during aging. The effects of senescence on morphological aspects in SCN are important for understanding some alterations in biological rhythms expression. Therefore, our aim was to perform a comparative study of the morphological aspects of SCN in adult and aged female marmoset. Morphometric analysis of SCN was performed using Nissl staining, NeuN-IR, GFAP-IR, and CB-IR. A significant decrease in the SCN cells staining with Nissl, NeuN, and CB were observed in aged female marmosets compared to adults, while a significant increase in glial cells was found in aged marmosets, thus suggesting compensatory process due to neuronal loss evoked by aging.
视交叉上核（SCN）被认为是哺乳动物的中枢昼夜节律起搏器。衰老动物表现出内部时间紊乱，这可能是由SCN组件的形态和神经化学变化引起的。一些研究报道了大鼠和非人灵长类动物衰老过程中SCN中神经元细胞和神经胶质细胞的变化。衰老对SCN形态方面的影响对于理解生物节律表达的一些改变很重要。因此，我们的目的是对成年和老年雌性狨猴的SCN形态方面进行比较研究。使用尼氏染色、NeuN免疫反应（NeuN-IR）、胶质纤维酸性蛋白免疫反应（GFAP-IR）和钙结合蛋白免疫反应（CB-IR）对SCN进行形态计量分析。与成年雌性狨猴相比，老年雌性狨猴中用尼氏染色、NeuN和CB染色的SCN细胞显著减少，而老年狨猴中胶质细胞显著增加，这表明衰老引起的神经元丢失导致了代偿过程。
