Pasricha Rashmi, Saini Neeraj K, Rathor Nisha, Pathak Rakesh, Sinha Rajesh, Varma-Basil Mandira, Mishra Kiran, Brahmachari Vani, Bose Mridula
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India.
Pathog Dis. 2014 Dec;72(3):188-96. doi: 10.1111/2049-632X.12200. Epub 2014 Aug 7.
Lipoproteins are known to be effective immunogens and affect both innate and adaptive immunity. The lprN gene of Mycobacterium tuberculosis has been predicted to encode for a putative lipoprotein in silico. Here, we studied its function as an immunogen by in vivo studies in mice. The recombinant LprN protein, expressed and purified in Escherichia coli, triggered a cell-mediated immune response in BALB/c mice. This was observed by significantly higher T-cell proliferation and increased production of TNF-α and IFN-γ cytokines. However, pre-exposure to LprN protein failed to provide protection in mice after challenge with a virulent strain of M. tuberculosis. Histological examination showed an increase in tissue destruction in experimental animals, indicating an immunogenic potential for LprN protein that enhanced the virulence of bacilli.
众所周知，脂蛋白是有效的免疫原，会影响先天性免疫和适应性免疫。结核分枝杆菌的lprN基因在计算机模拟中被预测编码一种假定的脂蛋白。在此，我们通过在小鼠体内进行的研究来探究其作为免疫原的功能。在大肠杆菌中表达并纯化的重组LprN蛋白在BALB/c小鼠中引发了细胞介导的免疫反应。这可通过显著更高的T细胞增殖以及肿瘤坏死因子-α和干扰素-γ细胞因子产量增加得以观察到。然而，在感染强毒株结核分枝杆菌之前预先接触LprN蛋白未能为小鼠提供保护。组织学检查显示实验动物的组织破坏增加，表明LprN蛋白具有增强杆菌毒力的免疫原性潜力。
