Chi Ying, Han Zhi-Bo, Xu Fang-Yun, Wang You-Wei, Feng Xiao-Ming, Chen Fang, Ma Feng-Xia, Du Wen-Jing, Han Zhong-Chao
State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
State Key Laboratory of Experimental Hematology, Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Jun;22(3):588-94. doi: 10.7534/j.issn.1009-2137.2014.03.003.
Mesenchymal stem cells (MSCs) could be obtained from many sources, and there are differences between them. This study was purposed to compare and analyze the basic biological characteristics of umbilical cord, adipose tissue-and bone marrow-derived MSC (UC-MSCs, AD-MSCs and BM-MSCs). The MSCs were isolated from umbilical cord, adipose tissue and bone marrow were cultured; the morphology of UC-MSCs, AD-MSCs and BM-MSCs was observed by using microscopy; the immunophenotype, differentiation potential and expression of peroxisome proliferation-activated receptor-γ (PPAR-γ) mRNA were detected by using flow cytometry, differentiation test (von kossais and 0:1 red O staining) and quantitative fluorescent PCR, respectively. The results showed that the UC-MSCs, AD-MSCs and BM-MSCs displayed similar morphology under confocal microscope after being stained with rhodamine phalloidin and DAPL. The immunophenotypes of these three originated cells conform to coincide with identification criterion for MSCs, and showed similar expression level. During adipogenic induction the adipogenic potential of these MSCs was different, AD-MSCs exhibited the highest adipogenic potential, UC-MSCs displayed the lowest, while potential of BM-MSCs get between; however, the osteogenic differentiation potential of UC-MSCs, AD-MSCs and BM-MSCs was similar. The PCR detection showed that the expression level of PPAR-γ mRNA was the highest in AD-MSCs and the lowest in UC-MSCs, while expression level in BM-MSCs get between, these results were identical with the adipogenic potential, suggest that the difference of adipogenic potential in 3 kinds of MSCs was associated with basic expression level of PPAR-γ mRNA. It is concluded that UC-MSCs, AD-MSCs and BM-MSCs exhibit similar morphology, the immunophenotypes of these MSCs coincide with identification criterion for MSCs, the osteogenic potential of these MSCs is similar, while the adipogenic potential and the expression level of PPAR-γ mRNA are different. The difference-associated mechanisms need to further study.
间充质干细胞（MSCs）可从多种来源获取，且它们之间存在差异。本研究旨在比较和分析脐带、脂肪组织和骨髓来源的间充质干细胞（UC-MSCs、AD-MSCs和BM-MSCs）的基本生物学特性。从脐带、脂肪组织和骨髓中分离出间充质干细胞并进行培养；通过显微镜观察UC-MSCs、AD-MSCs和BM-MSCs的形态；分别采用流式细胞术、分化试验（von kossais和油红O染色）和定量荧光PCR检测免疫表型、分化潜能及过氧化物酶体增殖激活受体-γ（PPAR-γ）mRNA的表达。结果显示，用罗丹明鬼笔环肽和DAPI染色后，UC-MSCs、AD-MSCs和BM-MSCs在共聚焦显微镜下呈现相似的形态。这三种来源细胞的免疫表型均符合间充质干细胞的鉴定标准，且表达水平相似。在成脂诱导过程中，这些间充质干细胞的成脂潜能不同，AD-MSCs的成脂潜能最高，UC-MSCs最低，BM-MSCs的潜能介于两者之间；然而，UC-MSCs、AD-MSCs和BM-MSCs的成骨分化潜能相似。PCR检测显示，PPAR-γ mRNA的表达水平在AD-MSCs中最高，在UC-MSCs中最低，BM-MSCs中的表达水平介于两者之间，这些结果与成脂潜能一致，表明三种间充质干细胞成脂潜能的差异与PPAR-γ mRNA的基础表达水平有关。结论是，UC-MSCs、AD-MSCs和BM-MSCs呈现相似的形态，这些间充质干细胞的免疫表型符合间充质干细胞的鉴定标准，它们的成骨潜能相似，而成脂潜能和PPAR-γ mRNA的表达水平不同。相关差异机制有待进一步研究。
