Honguntikar Sachin D, Uppangala Shubhashree, Salian Sujith R, Kalthur Guruprasad, Kumar Pratap, Adiga Satish K
Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, 576 104, India.
Lasers Med Sci. 2015 Jan;30(1):95-101. doi: 10.1007/s10103-014-1625-1. Epub 2014 Jul 3.
This study aims to investigate the influence of two- (day 2) and six-to-eight-cell-stage (day 3) laser-assisted hatchings on the developmental potential and genetic integrity of the embryos. In this prospective experimental study, two- and six-to-eight-cell-stage mouse embryos were subjected to laser hatching using 1,480 nm diode laser, and then assessed for the developmental potential and DNA integrity in blastocysts. Similarly, four-cell-stage human embryos from 20 patients were also subjected to laser hatching, and then assessed for the developmental competence. Laser-assisted hatching in mouse embryos significantly enhanced the blastocyst hatching potential on day 4.5 (P < 0.0001). However, a significant decline in blastocyst total cell number (TCN) was observed in six-to-eight-cell-stage laser-hatched embryos (P < 0.001). Conversely, no significant difference in TCN was observed between laser-hatched and unhatched human four-cell-stage embryos after 24 h. Attempt to understand the genetic integrity in laser-hatched mouse blastocysts revealed significantly higher labeling index when hatching was done at two- (P < 0.01) and six-to-eight-cell stage (P < 0.05). DNA damage induced by the laser manipulation may affect implantation and postimplantation developmental potential of the embryos. However, further studies are required to elucidate the impact of laser-induced DNA damage on the reproductive outcome.
本研究旨在调查二细胞期（第2天）和六至八细胞期（第3天）激光辅助孵化对胚胎发育潜能和遗传完整性的影响。在这项前瞻性实验研究中，使用1480纳米二极管激光对二细胞期和六至八细胞期的小鼠胚胎进行激光孵化，然后评估囊胚的发育潜能和DNA完整性。同样，对来自20名患者的四细胞期人类胚胎也进行激光孵化，然后评估其发育能力。小鼠胚胎的激光辅助孵化在第4.5天显著提高了囊胚孵化潜能（P < 0.0001）。然而，在六至八细胞期激光孵化的胚胎中观察到囊胚总细胞数（TCN）显著下降（P < 0.001）。相反，24小时后，激光孵化和未孵化的人类四细胞期胚胎之间的TCN没有显著差异。对激光孵化的小鼠囊胚遗传完整性的研究发现，在二细胞期（P < 0.01）和六至八细胞期（P < 0.05）进行孵化时，标记指数显著更高。激光操作引起的DNA损伤可能会影响胚胎的着床和着床后发育潜能。然而，需要进一步研究来阐明激光诱导的DNA损伤对生殖结局的影响。
