中国甘肃合垫刃线虫新种（线虫纲：短体线虫亚科）的形态学和分子特征

Morphological and molecular characterization of Zygotylenchus gansuensis n. sp. (Nematoda: Pratylenchinae) from China.

作者信息

Wang Honghong, Zhuo Kan, Liao Jinling

机构信息

Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, China Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China; Email: unknown.

出版信息

Zootaxa. 2014 Jun 24;3821(4):465-75. doi: 10.11646/zootaxa.3821.4.5.

DOI:10.11646/zootaxa.3821.4.5

PMID:24989759

Abstract

Zygotylenchus gansuensis n. sp. is described and illustrated from the rhizosphere of jujube (Zizyphus jujuba Mill.) based on morphology and molecular analyses. This new species is characterized by a low and flattened labial region with three annuli, stylet 14.1±0.5 (13.0-14.9) μm long, deirids absent, five lateral lines in the vulval region, pharyngeal glands overlapping ventrally or ventrolaterally, V = 56.4±1.6 (54.0-60.8), indistinct spermatheca, subcylindrical tail with smooth and rounded terminus, and males absent. Molecular analyses show that the species has unique partial SSU, LSU D2D3 and ITS rRNA sequences. Phylogenetic relationships of Z. gansuensis n. sp. with other nematodes in Pratylenchinae are analysed using SSU and LSU D2D3.

摘要

基于形态学和分子分析，对采自枣树（Zizyphus jujuba Mill.）根际的甘肃合垫刃线虫新种（Zygotylenchus gansuensis n. sp.）进行了描述和图示。该新种的特征为：唇区低平，具3个环纹；口针长14.1±0.5（13.0 - 14.9）μm；排泄孔缺失；阴门区有5条侧线；咽腺在腹面或腹侧面重叠；V值为56.4±1.6（54.0 - 60.8）；受精囊不明显；尾部亚圆柱形，末端光滑且圆；无雄虫。分子分析表明，该物种具有独特的部分小亚基（SSU）、大亚基D2D3（LSU D2D3）和内转录间隔区（ITS）核糖体RNA序列。利用SSU和LSU D2D3分析了甘肃合垫刃线虫新种与短体线虫亚科其他线虫的系统发育关系。