Kamiya Yasutaka, Yamazaki Kenji, Ogino Toshio
Yokohama National University, 79-5, Tokiwadai, Hodogaya, Yokohama 240-8501, Japan.
Yokohama National University, 79-5, Tokiwadai, Hodogaya, Yokohama 240-8501, Japan.
J Colloid Interface Sci. 2014 Oct 1;431:77-81. doi: 10.1016/j.jcis.2014.06.023. Epub 2014 Jun 19.
We have investigated effects of the support substrate surfaces on properties of the attached graphene flakes by observing protein adsorption to the graphene surfaces on SiO2/Si substrates that are modified with self-assembled monolayers to control their hydrophilicity. Using atomic force microscopy operated in aqueous environment, we found that high-density clusters of agglomerated avidin molecules form on the graphene flakes in the areas supported by a hydrophobic substrate surface, whereas very low density of large avidin clusters form at the edge of graphene flakes in the area supported by a hydrophilic surface. These results demonstrate that hydrophilicity of the support surface affects hydrophilicity of the graphene surface also in aqueous environment and that surface modification of the support substrate is a useful technique to control protein adsorption phenomena on graphene surfaces for realization of high sensitive graphene biosensors.
我们通过观察蛋白质在经自组装单分子层修饰以控制其亲水性的SiO₂/Si衬底上的石墨烯表面的吸附情况，研究了支撑衬底表面对附着的石墨烯薄片性质的影响。利用在水环境中操作的原子力显微镜，我们发现，在疏水衬底表面支撑的区域，石墨烯薄片上会形成高密度的聚集抗生物素蛋白分子簇，而在亲水表面支撑的区域，石墨烯薄片边缘形成的大抗生物素蛋白簇的密度则非常低。这些结果表明，在水环境中，支撑表面的亲水性也会影响石墨烯表面的亲水性，并且支撑衬底的表面修饰是一种用于控制石墨烯表面蛋白质吸附现象以实现高灵敏度石墨烯生物传感器的有用技术。
