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小鼠卵巢癌中GPC3的表达通过M1巨噬细胞诱导GPC3特异性T细胞介导的免疫反应并抑制肿瘤生长。

GPC3 expression in mouse ovarian cancer induces GPC3‑specific T cell-mediated immune response through M1 macrophages and suppresses tumor growth.

作者信息

Luo Chenhong, Shibata Kiyosumi, Suzuki Shiro, Kajiyama Hiroaki, Senga Takeshi, Koya Yoshihiro, Daimon Mina, Yamashita Mamoru, Kikkawa Fumitaka

机构信息

Bio-Databases Institute of Reproductive and Developmental Medicine, Nagoya 458-0818, Japan.

Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Nagoya 466‑8550, Japan.

出版信息

Oncol Rep. 2014 Sep;32(3):913-21. doi: 10.3892/or.2014.3300. Epub 2014 Jul 2.

DOI:10.3892/or.2014.3300
PMID:24992906
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4121400/
Abstract

Glypican-3 (GPC3) is specifically expressed in ovarian clear cell carcinoma (OCCC), hepatocellular carci-noma (HCC), and melanoma and lung cancer. GPC3 is being explored as a potential candidate for OCCC and HCC immunotherapy. As a tumor-associated antigen, induction of immune response of GPC3 in ovarian cancer remains elusive. We established a GPC3 transgenic mouse ovarian cancer cell line, OV2944-HM-1 (HM-1), and used the intraperitoneal ovarian cancer mouse model to investigate immune response in GPC3-expressing tumor. We found that GPC3 expression in the tumor increased F4/80+CD86+ macrophage (M1) proportion and caused GPC3-specific CD8+ T cell immune responses, and prolonged mouse survival. Our results demonstrated that GPC3 expression induced T cell-mediated immune response in this mouse ovarian cancer model and also provided supportive evidence that GPC3 is an ideal target for ovarian cancer immunotherapy.

摘要

磷脂酰肌醇蛋白聚糖-3（GPC3）在卵巢透明细胞癌（OCCC）、肝细胞癌（HCC）、黑色素瘤和肺癌中特异性表达。GPC3正作为OCCC和HCC免疫治疗的潜在候选物进行研究。作为一种肿瘤相关抗原，GPC3在卵巢癌中诱导免疫反应的机制仍不清楚。我们建立了一种GPC3转基因小鼠卵巢癌细胞系OV2944-HM-1（HM-1），并使用腹腔内卵巢癌小鼠模型来研究表达GPC3的肿瘤中的免疫反应。我们发现肿瘤中GPC3的表达增加了F4/80+CD86+巨噬细胞（M1）的比例，并引发了GPC3特异性CD8+T细胞免疫反应，延长了小鼠的生存期。我们的结果表明，在该小鼠卵巢癌模型中，GPC3的表达诱导了T细胞介导的免疫反应，也为GPC3是卵巢癌免疫治疗的理想靶点提供了支持性证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e7/4121400/ea83a0639531/OR-32-03-0913-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e7/4121400/f6a554bf8705/OR-32-03-0913-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e7/4121400/006fdb83c2ea/OR-32-03-0913-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e7/4121400/d9bcfae7bb01/OR-32-03-0913-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e7/4121400/45652ce3c102/OR-32-03-0913-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e7/4121400/ea83a0639531/OR-32-03-0913-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e7/4121400/f6a554bf8705/OR-32-03-0913-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e7/4121400/006fdb83c2ea/OR-32-03-0913-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e7/4121400/d9bcfae7bb01/OR-32-03-0913-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e7/4121400/45652ce3c102/OR-32-03-0913-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e7/4121400/ea83a0639531/OR-32-03-0913-g04.jpg

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2
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Int J Oncol. 2012 Nov;41(5):1601-9. doi: 10.3892/ijo.2012.1626. Epub 2012 Sep 11.
3
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4
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5
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7
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