丹参酮II - A对暴露于子痫前期患者血清后的人脐静脉内皮细胞损伤具有保护作用。
Tanshinone II-A is protective against human umbilical vein endothelial cell injury after exposure to serum from preeclampsia patients.
作者信息
Wu ChunFeng, Yuan Jing, Sui RenFang, Li ShuYuan, Sun JingXia
机构信息
Department of Obstetrics and Gynecology, First Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin, China.
出版信息
Gynecol Obstet Invest. 2014;78(2):101-8. doi: 10.1159/000363294. Epub 2014 Jul 2.
BACKGROUND/AIMS: Preeclampsia (PE) is one of the most common and dangerous complications during pregnancy and is characterized by high blood pressure and significant amounts of protein in the urine. Vascular endothelial cell dysfunction is the major pathology in PE. This study was designed to assay the effects of tanshinone II-A (TII-A) on human umbilical vein endothelial cell (HUVEC) injury after incubation with serum from PE patients and to determine the underlying mechanism.
METHODS
After treating HUVECs with different TII-A concentrations, cell viability, apoptosis and CD40/CD40 ligand (CD40L) mRNA and protein expression levels were measured.
RESULTS
Incubation of HUVECs with serum from PE patients induced morphological alterations, caused decreased cell viability and increased the rate of apoptosis. However, TII-A (5-40 μg/ml) significantly reversed these injuries. Importantly, preapplication of TII-A attenuated PE sera-induced expression of CD40 and CD40L mRNA and protein.
CONCLUSION
TII-A has a protective effect against PE sera, likely through regulation of the CD40/CD40L signal transduction pathway.
背景/目的：子痫前期（PE）是孕期最常见且危险的并发症之一，其特征为高血压和大量蛋白尿。血管内皮细胞功能障碍是子痫前期的主要病理表现。本研究旨在检测丹参酮II - A（TII - A）对人脐静脉内皮细胞（HUVEC）在与子痫前期患者血清孵育后的损伤作用，并确定其潜在机制。
方法
用不同浓度的TII - A处理人脐静脉内皮细胞后，检测细胞活力、凋亡以及CD40/CD40配体（CD40L）的mRNA和蛋白表达水平。
结果
将人脐静脉内皮细胞与子痫前期患者血清孵育可诱导形态学改变，导致细胞活力下降并增加凋亡率。然而，TII - A（5 - 40μg/ml）可显著逆转这些损伤。重要的是，预先应用TII - A可减弱子痫前期血清诱导的CD40和CD40L mRNA及蛋白表达。
结论
TII - A对子痫前期血清具有保护作用，可能是通过调节CD40/CD40L信号转导通路实现的。
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