Bukholm G, Nedenskov-Sørensen P, Bøvre K
Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Rikshospitalet, Norway.
APMIS. 1989 May;97(5):472-4.
DNA-DNA hybridization in solution was used to characterize 23 human isolates of Campylobacter pylori. The 23 isolates showed DNA affinity with the type strain (NCTC 11637). The relative binding ratios varied between 0.83 and 1. Type strains of C. coli (NCTC 11366), C. jejuni (NCTC 11351), C. laridis (NCTC 11352), C. sputorum subsp. sputorum (ATCC 35980), Wolinella recta (NCTC 11489) and W. succinogenes (ATCC 29543) showed relative binding ratios less than 0.01 compared to the C. pylori type strain. The results suggest that C. pylori is a homogenous taxonomic unit distinctly separated from these other species.
采用溶液中的DNA-DNA杂交技术对23株人幽门螺杆菌分离株进行特征分析。这23株分离株显示出与模式菌株(NCTC 11637)有DNA亲和力。相对结合率在0.83至1之间变化。大肠杆菌(NCTC 11366)、空肠弯曲菌(NCTC 11351)、拉氏弯曲菌(NCTC 11352)、唾液弯曲菌唾液亚种(ATCC 35980)、直肠沃氏菌(NCTC 11489)和产琥珀酸沃氏菌(ATCC 29543)的模式菌株与幽门螺杆菌模式菌株相比,相对结合率小于0.01。结果表明,幽门螺杆菌是一个与其他这些物种明显分开的同质分类单元。