Center for Reproductive Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
Stem Cells and Reprogramming Group, Biological Engineering Laboratories, Monash University, Clayton, Victoria, Australia.
Fertil Steril. 2014 Sep;102(3):871-877.e3. doi: 10.1016/j.fertnstert.2014.05.040. Epub 2014 Jun 30.
To investigate the antiapoptosis effect of sphingosine-1-phosphate (S1P) on human fetal ovarian tissue treated by cyclophosphamide (CTX).
Experimental animal study.
University center for reproductive medicine and IVF unit.
ANIMAL(S): Female immunodeficient BALB/c nude mice, 6 to 8 weeks old.
INTERVENTION(S): Human fetal ovarian tissue slowly cryopreserved then subcutaneously transplanted in immunodeficient mice.
MAIN OUTCOME MEASURE(S): Follicle survival assessed qualitatively and quantitatively using H&E staining, and cellular apoptosis of the ovarian grafts evaluated using transmission electron microscopy and DNA nick end labeling in situ (TUNEL assay).
RESULT(S): The alkylating agent CTX caused a substantial follicle loss and apoptotic DNA fragmentation in the ovarian grafts in a period of 2 weeks of transplantation. The S1P treatment significantly prevented follicular apoptosis and maintained primordial follicle population in the grafts.
CONCLUSION(S): This study shows for the first time that S1P protects primordial follicles in human ovarian grafts from a chemotherapy drug treatment via suppressing follicle apoptosis.
探讨神经鞘氨醇-1-磷酸（S1P）对环磷酰胺（CTX）处理的人胎儿卵巢组织的抗凋亡作用。
实验动物研究。
大学生殖医学中心和体外受精单位。
6 至 8 周龄的雌性免疫缺陷 BALB/c 裸鼠。
人胎儿卵巢组织慢速冻存，然后皮下移植到免疫缺陷小鼠中。
使用 H&E 染色定性和定量评估卵泡存活，并用透射电子显微镜和原位 DNA 缺口末端标记（TUNEL 测定）评估卵巢移植物的细胞凋亡。
烷化剂 CTX 在移植后 2 周内导致卵巢移植物中大量卵泡丢失和凋亡性 DNA 片段化。S1P 处理可显著防止卵泡凋亡，维持移植物中原始卵泡的数量。
本研究首次表明，S1P 通过抑制卵泡凋亡来保护人卵巢移植物中的原始卵泡免受化疗药物的影响。
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