根据临床结果比较智利极幼龄婴儿急性下呼吸道感染中呼吸道合胞病毒和鼻病毒的病毒学特征。

Comparison of virological profiles of respiratory syncytial virus and rhinovirus in acute lower tract respiratory infections in very young Chilean infants, according to their clinical outcome.

作者信息

Luchsinger Vivian, Ampuero Sandra, Palomino M Angélica, Chnaiderman Jonás, Levican Jorge, Gaggero Aldo, Larrañaga Carmen E

机构信息

Program of Virology of the Institute of Science in Biomedicine, Faculty of Medicine, University of Chile, Santiago, Chile.

Department of Pediatrics, Hospital Roberto del Río, Faculty of Medicine, University of Chile, Santiago, Chile.

出版信息

J Clin Virol. 2014 Sep;61(1):138-44. doi: 10.1016/j.jcv.2014.06.004. Epub 2014 Jun 13.

DOI:10.1016/j.jcv.2014.06.004

PMID:24994006

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7185600/

Abstract

BACKGROUND

Respiratory syncytial virus (RSV) and rhinovirus (HRV) are the main cause of acute lower respiratory tract infections (ALRTIs) in infants. Viral and host-related risk factors for severe disease have also not been clearly established.

OBJECTIVE

To assess whether certain viral features of RSV and, or HRV are associated with severe ALRTI.

STUDY DESIGN

RSV and HRV were studied in nasopharyngeal samples of infants by immunofluorescence, Luminex(®) and/or real-time RT-PCR assays. Quantitation and genotyping of RSV and HRV by PCR were done.

RESULTS

Of 124 virus positive specimens, 74 (59.7%) had RSV; 22 (17.7%) HRV and 28 (22.6%) RSV-HRV co-infection. Hospitalization was required in 57/74 RSV infants (77.0%); in 10/22 HRV cases (45.5%) (p=0.006) and in 15/28 co-infected by both viruses (53.6%) (p=0.003). Severe cases were 33/74 (44.6%) RSV infections, 2/22 HRV cases (9.1%), (p<0.002) and 6/28 (21.4%) patients co-infected by RSV-HRV (p<0.026). Three genotypes (NA1, B7, B9) of RSV circulated during the study. In 33 severe infants, NA1 was detected in 19 cases (57.6%); B7 in 13 (39.4%) and B9 in 1 (3.0%) (p<0.01; OR=10.0). RSV loads were similar between outpatients and hospitalized infants (p=0.7) and among different severities (p=0.7). NA1 loads were higher than other strains (p=0.049). Three geno-groups of HRV circulated homogeneously.

CONCLUSION

In very young infants, RSV cause more severe disease than HRV. Co-infection does not increase the severity of illness. NA1 RSV genotype was associated with major frequency of hospitalization, severe respiratory disease and higher viral load.

摘要

背景

呼吸道合胞病毒（RSV）和鼻病毒（HRV）是婴儿急性下呼吸道感染（ALRTIs）的主要病因。严重疾病的病毒相关和宿主相关危险因素也尚未明确确立。

目的

评估RSV和/或HRV的某些病毒特征是否与严重ALRTI相关。

研究设计

通过免疫荧光、Luminex（®）和/或实时逆转录聚合酶链反应（RT-PCR）检测法对婴儿鼻咽样本中的RSV和HRV进行研究。通过聚合酶链反应（PCR）对RSV和HRV进行定量和基因分型。

结果

在124份病毒阳性标本中，74份（59.7%）为RSV；22份（17.7%）为HRV，28份（22.6%）为RSV-HRV合并感染。74例RSV感染婴儿中有57例（77.0%）需要住院治疗；22例HRV感染病例中有10例（45.5%）（p = =0.006），两种病毒合并感染的病例中有15例（53.6%）（p = 0.003）。严重病例中，RSV感染有33例（44.6%），HRV感染有2例（9.1%）（p<0.002）；RSV-HRV合并感染患者中有6例（21.4%）（p<0.026）。研究期间有三种RSV基因型（NA1、B7、B9）传播。在33例重症婴儿中，检测到NA1型的有19例（57.6%）；B7型13例（39.4%），B9型1例（3.0%）（p<0.01；比值比（OR = 10.0）。门诊婴儿和住院婴儿的RSV载量相似（p = 0.7），不同严重程度的婴儿之间也相似（p = 0.7）。NA1型的载量高于其他毒株（p = 0.049）。HRV的三个基因群均匀传播。