Zhang Junbo, Yin Shuanghong, Guo Fei, Meng Ren, Chen Chuangfu, Zhang Hui, Li Zhiqiang, Fu Qiang, Shi Huijun, Hu Shengwei, Ni Wei, Li Tiansen, Zhang Ke
College of Animal Science and Technology, Shihezi University, Shihezi, 832003, P. R. China.
J Microbiol. 2014 Aug;52(8):681-8. doi: 10.1007/s12275-014-3689-9. Epub 2014 Jul 4.
Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Δery) was constructed to overcome these drawbacks. The growth of the Δery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Δery induced an anti-Brucella-specific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-γ) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Δery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.
布鲁氏菌病是一种全球分布的人畜共患病，可导致动物和人类发病。然而，目前的牛布鲁氏菌疫苗（S19和RB51）存在缺陷；它们可导致怀孕动物流产。此外，使用疫苗S19时，检测无法区分自然感染和疫苗接种感染。因此，需要一种更安全、效力更强的疫苗。构建了牛布鲁氏菌2308 ery启动子突变体（Δery）以克服这些缺点。Δery突变体在巨噬细胞和小鼠中的生长显著减弱，并在小鼠中诱导了高度的保护性免疫。此外，Δery诱导了抗布鲁氏菌特异性IgG（免疫球蛋白G）反应，并刺激了干扰素-γ（INF-γ）和白细胞介素-4（IL-4）的表达。此外，EryA抗原的表达使得能够在小鼠中区分自然感染和疫苗接种感染。这些结果表明，Δery突变体是一种潜在的减毒活疫苗候选物，可用于预防强毒牛布鲁氏菌2308（S2308）感染。
