miR-126 和 miR-20b 在遗传性和非遗传性结直肠肿瘤中的低表达。

Underexpression of miR-126 and miR-20b in hereditary and nonhereditary colorectal tumors.

机构信息

Department of Surgery, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Tokyo, Japan.

出版信息

Oncology. 2014;87(1):58-66. doi: 10.1159/000363303. Epub 2014 Jun 28.

DOI:10.1159/000363303

PMID:24994098

Abstract

OBJECTIVES

The aim of the study was to determine the significance of miR-126 and miR-20b in colorectal carcinogenesis.

METHODS

We analyzed the expressions of miR-126 and miR-20b in 136 colorectal tumors from 39 microsatellite stable (MSS) tumors, 23 high microsatellite instability (MSI-H) tumors, 16 Lynch syndrome, and 58 familial adenomatous polyposis (FAP) tumors including adenoma, intramucosal carcinoma, and invasive carcinoma.

RESULTS

All four kinds of tumors showed underexpression of both miR-126 and miR-20b. The frequency of miR-126 downregulation was 100.0% in FAP adenomas, 85.7% in FAP intramucosal carcinomas, 78.9% in invasive carcinomas, 81.3% in Lynch syndrome tumors, 68.4% in MSS tumors, and 65.4% in MSI-H tumors. The frequency of miR-20b downregulation was 64.0% in FAP adenomas, 50.0% in FAP intramucosal carcinomas, 73.3% in invasive carcinomas, 62.5% in Lynch syndrome tumors, 79.5% in MSS tumors, and 91.3% in MSI-H tumors. The current study demonstrated underexpression of miR-126 and miR-20b in various types of colorectal cancer. These findings support the hypothesis that angiogenesis results from underexpressions of miR-126 and miR-20b and occurs as an early event in colorectal carcinogenesis.

CONCLUSIONS

Underexpression of miR-126 and miR-20b was observed in various types of colorectal cancer, and occurs as an early event of colorectal carcinogenesis in FAP tumors.

摘要

目的

本研究旨在探讨 miR-126 和 miR-20b 在结直肠癌发生发展中的意义。

方法

我们分析了 136 例结直肠肿瘤（39 例微卫星稳定型（MSS）肿瘤、23 例高微卫星不稳定性（MSI-H）肿瘤、16 例林奇综合征和 58 例家族性腺瘤性息肉病（FAP）肿瘤）中 miR-126 和 miR-20b 的表达情况，这些肿瘤包括腺瘤、黏膜内癌和浸润性癌。

结果

所有四种类型的肿瘤均表现出 miR-126 和 miR-20b 的低表达。FAP 腺瘤中 miR-126 下调的频率为 100.0%，FAP 黏膜内癌为 85.7%，浸润性癌为 78.9%，林奇综合征肿瘤为 81.3%，MSS 肿瘤为 68.4%，MSI-H 肿瘤为 65.4%。miR-20b 下调的频率在 FAP 腺瘤中为 64.0%，FAP 黏膜内癌为 50.0%，浸润性癌为 73.3%，林奇综合征肿瘤为 62.5%，MSS 肿瘤为 79.5%，MSI-H 肿瘤为 91.3%。本研究表明，miR-126 和 miR-20b 在各种类型的结直肠癌中表达下调。这些发现支持这样一种假说，即血管生成是由于 miR-126 和 miR-20b 的表达下调而产生的，并且是结直肠癌发生的早期事件。