Malagón M M, García-Navarro S, Ruiz-Navarro A, Gracia-Navarro F
Department of Cell Biology, University of Córdoba, Spain.
Cell Tissue Res. 1989;256(2):391-8. doi: 10.1007/BF00218897.
The effects of synthetic thyrotropin-releasing hormone on pituitary prolactin and thyrotropic cells were investigated in adult male Rana perezi (formerly Rana ridibunda) frogs. Animals were given daily injections of synthetic thyrotropin-releasing hormone into the dorsal lymph sac. Prolactin and thyrotropic cells were identified by the colloidal-gold method, using anti-human prolactin and anti-human-beta-thyrotropin hormone as primary antisera. The stereological parameters of the rough endoplasmic reticulum, Golgi complex, and secretory granules of prolactin and thyrotropic cells were evaluated by ultrastructural morphometry (point-counting method). Thyrotropin-releasing hormone caused cytological changes in both cell-types which were consistent with increased synthesis and release of both prolactin and thyrotropin. These changes were still significant after 48 h treatment in the case of thyrotropic cells, while in prolactin cells the thyrotropin-releasing hormone increased the number of secretory granules. After 6 days, the cells resembled essentially those used as controls. These results indicate that thyrotropin-releasing hormone stimulates the synthesis and release of prolactin and thyrotropin, and that the response of each cell type to this hypothalamic stimulus follows a different time-course.
在成年雄性佩雷斯蛙（原泽蛙）中研究了合成促甲状腺激素释放激素对垂体催乳素细胞和促甲状腺细胞的影响。每天将合成促甲状腺激素释放激素注射到动物的背部淋巴囊中。使用抗人催乳素和抗人β - 促甲状腺激素作为一抗，通过胶体金法鉴定催乳素细胞和促甲状腺细胞。通过超微结构形态计量学（点计数法）评估催乳素细胞和促甲状腺细胞的粗面内质网、高尔基体复合体和分泌颗粒的立体学参数。促甲状腺激素释放激素引起两种细胞类型的细胞学变化，这与催乳素和促甲状腺激素合成与释放增加一致。对于促甲状腺细胞，在处理48小时后这些变化仍然显著，而在催乳素细胞中促甲状腺激素释放激素增加了分泌颗粒的数量。6天后，这些细胞基本上类似于用作对照的细胞。这些结果表明促甲状腺激素释放激素刺激催乳素和促甲状腺激素的合成与释放，并且每种细胞类型对这种下丘脑刺激的反应遵循不同的时间进程。
