Anizan Sébastien, Bergamaschi Mateus M, Barnes Allan J, Milman Garry, Desrosiers Nathalie, Lee Dayong, Gorelick David A, Huestis Marilyn A
Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, 251 Bayview Blvd, Suite 200, Baltimore, MD, 21224, USA.
Drug Test Anal. 2015 Feb;7(2):114-20. doi: 10.1002/dta.1688. Epub 2014 Jul 3.
Evaluation of cannabinoid stability in authentic oral fluid (OF) is critical, as most OF stability studies employed fortified or synthetic OF. Participants (n = 16) smoked a 6.8% delta-9-tetrahydrocannabinol (THC) cigarette, and baseline concentrations of THC, 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), and cannabinol (CBN) were determined within 24 h in 16 separate pooled samples (collected 1 h before to 10.5 or 13 h after smoking). OF was collected with the StatSure Saliva Sampler™ and Oral-Eze® devices. Oral-Eze samples were re-analyzed after room temperature (RT) storage for 1 week, and for both devices after 4 °C for 1 and 4 weeks, and -20 °C for 4 and 24 weeks. Concentrations ±20% from initial concentrations were considered stable. With the StatSure device, all cannabinoids were within 80-120% median %baseline for all storage conditions. Individual THC, CBD, CBN and THCCOOH pool concentrations were stable in 100%, 100%, 80-94% and >85%, respectively, across storage conditions. With the Oral-Eze device, at RT or refrigerated storage (for 1 and 4 weeks), THC, CBD and THCCOOH were stable in 94-100%, 78-89%, and 93-100% of samples, respectively, while CBN concentrations were 53-79% stable. However, after 24 weeks at -20 °C, stability decreased, especially for CBD, with a median of 56% stability. Overall, the collection devices' elution/stabilizing buffers provided good stability for OF cannabinoids, with the exception of the more labile CBN. To ensure OF cannabinoid concentration accuracy, these data suggest analysis within 4 weeks at 4 °C storage for Oral-Eze collection and within 4 weeks at 4 °C or 24 weeks at -20 °C for StatSure collection. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.
评估真实口腔液（OF）中大麻素的稳定性至关重要，因为大多数口腔液稳定性研究使用的是强化或合成口腔液。16名参与者吸食了一支含6.8% Δ⁹-四氢大麻酚（THC）的香烟，并在24小时内对16个单独的混合样本（在吸烟前1小时至吸烟后10.5或13小时收集）测定了THC、11-去甲-9-羧基-THC（THCCOOH）、大麻二酚（CBD）和大麻酚（CBN）的基线浓度。使用StatSure唾液采样器™和Oral-Eze®装置收集口腔液。Oral-Eze样本在室温（RT）储存1周后重新分析，两种装置的样本在4℃分别储存1周和4周后以及在-20℃分别储存4周和24周后重新分析。与初始浓度相差±20%的浓度被视为稳定。使用StatSure装置，在所有储存条件下，所有大麻素的浓度均在中位数基线的80 - 120%范围内。在所有储存条件下，单个THC、CBD、CBN和THCCOOH混合样本的浓度稳定性分别为100%、100%、80 - 94%和>85%。使用Oral-Eze装置，在室温或冷藏储存（1周和4周）时，THC、CBD和THCCOOH在样本中的稳定性分别为94 - 100%、78 - 89%和93 - 100%，而CBN浓度的稳定性为53 - 79%。然而，在-20℃储存24周后，稳定性下降，尤其是CBD，中位数稳定性为56%。总体而言，除了更不稳定的CBN外，收集装置的洗脱/稳定缓冲液为口腔液中的大麻素提供了良好的稳定性。为确保口腔液中大麻素浓度的准确性，这些数据表明，对于Oral-Eze收集的样本，在4℃储存4周内进行分析；对于StatSure收集的样本，在4℃储存4周内或在-20℃储存24周内进行分析。2014年发表。本文为美国政府作品，在美国属于公共领域。
