Matthews James C, Zhang Zhi, Patterson Jennifer D, Bridges Phillip J, Stromberg Arnold J, Boling J A
Department of Animal and Food Sciences, University of Kentucky, Lexington, KY, 40546, USA,
Biol Trace Elem Res. 2014 Sep;160(3):321-39. doi: 10.1007/s12011-014-0050-4. Epub 2014 Jul 5.
Selenium (Se) is an important trace mineral that, due to deficiencies in the soil in many parts of the USA, must be supplemented directly to the diet of foraging cattle. Both organic and inorganic forms of dietary Se supplements are available and commonly used, and it is known that Se form affects tissue assimilation, bioavailability, and physiological responses. However, little is known about the effects of form of dietary Se supplements on gene expression profiles, which ostensibly account for Se form-dependent physiological processes. To determine if hepatic transcriptomes of growing beef (Angus-cross) heifers (0.5 kg gain/day) was altered by form of dietary supplemental Se, none (Control), or 3 mg Se/day as inorganic Se (ISe, sodium selenite), organic (OSe, Sel-Plex®), or a blend of ISe and OSe (1.5 mg:1.5 mg, Mix) Se was fed for 168 days, and the RNA expression profiles from biopsied liver tissues was compared by microarray analysis. The relative abundance of 139 RNA transcripts was affected by Se treatment, with 86 of these with complete gene annotations. Statistical and bioinformatic analysis of the annotated RNA transcripts revealed clear differences among the four Se treatment groups in their hepatic expression profiles, including (1) solely and commonly affected transcripts; (2) Control and OSe profiles being more similar than Mix and ISe treatments; (3) distinct OSe-, Mix-, and ISe-Se treatment-induced "phenotypes" that possessed both common and unique predicted physiological capacities; and (4) expression of three microRNAs were uniquely sensitive to OSe, ISe, or Mix treatments, including increased capacity for redox potential induced by OSe and Mix Se treatments resulting from decreased expression of MiR2300b messenger RNA. These findings indicate that the form of supplemental dietary Se consumed by cattle will affect the composition of liver transcriptomes resulting, presumably, in different physiological capacities.
硒（Se）是一种重要的微量矿物质，由于美国许多地区土壤中缺乏这种元素，必须直接添加到放牧牛的饮食中。膳食硒补充剂有有机和无机两种形式，且都常用，已知硒的形式会影响组织同化、生物利用度和生理反应。然而，关于膳食硒补充剂的形式对基因表达谱的影响却知之甚少，而基因表达谱表面上决定了硒形式依赖的生理过程。为了确定生长中的肉用（安格斯杂交）小母牛（日增重0.5千克）的肝脏转录组是否会因膳食补充硒的形式而改变，分别给小母牛饲喂无硒（对照）、或每天3毫克无机硒（ISe，亚硒酸钠）、有机硒（OSe，Sel-Plex®）、或无机硒与有机硒的混合物（1.5毫克:1.5毫克，混合），持续168天，然后通过微阵列分析比较活检肝脏组织的RNA表达谱。139种RNA转录本的相对丰度受硒处理的影响，其中86种具有完整的基因注释。对注释后的RNA转录本进行统计和生物信息学分析，结果显示四个硒处理组的肝脏表达谱存在明显差异，包括：（1）单独和共同受影响的转录本；（2）对照和OSe谱比混合和ISe处理更相似；（3）不同的OSe、混合和ISe硒处理诱导的“表型”，它们具有共同和独特的预测生理能力；（4）三种微小RNA的表达对OSe、ISe或混合处理具有独特的敏感性，包括由于MiR2300b信使核糖核酸表达降低导致OSe和混合硒处理诱导的氧化还原电位增加。这些发现表明，牛摄入的膳食补充硒的形式会影响肝脏转录组的组成，可能会导致不同的生理能力。
