Genetics Program, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
Genetics Program, Instituto Nacional de Câncer, Rio de Janeiro, Brazil; Genetics Department, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Leuk Res. 2014 Aug;38(8):983-7. doi: 10.1016/j.leukres.2014.06.001. Epub 2014 Jun 12.
Resistance to chemotherapy is one of the most relevant aspects of treatment failure in cancer. Cell lines are used as models to study resistance. We analyzed the transcriptional profile of two multidrug resistant (MDR) cell lines (Lucena 1 and FEPS) derived from the same drug-sensitive cell K562. Microarray data identified 130 differentially expressed genes (DEG) between K562 vs. Lucena 1, 1932 between K562 vs. FEPS, and 1211 between Lucena 1 versus FEPS. The NOTCH pathway was affected in FEPS with overexpression of NOTCH2 and HEY1. The highly overexpressed gene in MDR cell lines was ABCB1, and both presented the ABCB1 promoter unmethylated.
化疗耐药是癌症治疗失败的最相关方面之一。细胞系被用作研究耐药性的模型。我们分析了源自同一药物敏感细胞系 K562 的两种多药耐药 (MDR) 细胞系 (Lucena 1 和 FEPS) 的转录谱。微阵列数据鉴定出 K562 与 Lucena 1 之间的 130 个差异表达基因 (DEG)，K562 与 FEPS 之间的 1932 个，Lucena 1 与 FEPS 之间的 1211 个。NOTCH 途径在 FEPS 中受到影响，NOTCH2 和 HEY1 过表达。在 MDR 细胞系中高度过表达的基因是 ABCB1，并且两者均呈现 ABCB1 启动子未甲基化。
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