人慢性髓性白血病细胞系中多药耐药（MDR）与干细胞标志物之间的关系。
Relationships between multidrug resistance (MDR) and stem cell markers in human chronic myeloid leukemia cell lines.
机构信息
Programa de Pós-Graduação em Ciências Fisiológicas, Fisiologia Animal, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil.
出版信息
Leuk Res. 2010 Jun;34(6):757-62. doi: 10.1016/j.leukres.2009.11.004. Epub 2009 Dec 6.
The K562 cell line (chronic myeloid leukemia), sensitive to chemotherapy (non-MDR), and the Lucena cell line, resistant to chemotherapy (MDR) were investigated. The results suggest that both cell lines possess CD34+CD38- profiles of hematopoietic stem cell markers. The promoter regions of ABCB1, ABCG2 and ABCC1 genes contain binding sites for the Oct-4 transcripton factor, which is also considered a marker of tumor stem cells. Lucena cells showed an over-expression of the ABCB1 gene and a high expression of the Oct-4, ABCG2 and ABCC1 genes as compared to K562 cells.
我们研究了对化疗敏感（非多药耐药性，MDR）的 K562 细胞系（慢性髓性白血病）和对化疗耐药（多药耐药性，MDR）的 Lucena 细胞系。结果表明，这两种细胞系均具有造血干细胞标志物 CD34+CD38-的特征。ABCB1、ABCG2 和 ABCC1 基因的启动子区域包含结合转录因子 Oct-4 的结合位点，而 Oct-4 也被认为是肿瘤干细胞的标志物。与 K562 细胞相比，Lucena 细胞中 ABCB1 基因的过表达以及 Oct-4、ABCG2 和 ABCC1 基因的高表达更为明显。
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