用于研究微生物致病机制的先进质谱技术。
Advanced mass spectrometry technologies for the study of microbial pathogenesis.
作者信息
Moore Jessica L, Caprioli Richard M, Skaar Eric P
机构信息
Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, United States.
Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, United States.
出版信息
Curr Opin Microbiol. 2014 Jun;19:45-51. doi: 10.1016/j.mib.2014.05.023. Epub 2014 Jul 3.
Abstract
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has been successfully applied to the field of microbial pathogenesis with promising results, principally in diagnostic microbiology to rapidly identify bacteria based on the molecular profiles of small cell populations. Direct profiling of molecules from serum and tissue samples by MALDI MS provides a means to study the pathogen-host interaction and to discover potential markers of infection. Systematic molecular profiling across tissue sections represents a new imaging modality, enabling regiospecific molecular measurements to be made in situ, in both two-dimensional and three-dimensional analyses. Herein, we briefly summarize work that employs MALDI MS to study the pathogenesis of microbial infection.
摘要
基质辅助激光解吸/电离质谱（MALDI MS）已成功应用于微生物致病机制领域，并取得了令人鼓舞的成果，主要应用于诊断微生物学，以便根据小细胞群体的分子谱快速鉴定细菌。通过MALDI MS对血清和组织样本中的分子进行直接分析，为研究病原体与宿主的相互作用以及发现潜在的感染标志物提供了一种手段。跨组织切片的系统分子分析代表了一种新的成像方式，能够在二维和三维分析中进行原位区域特异性分子测量。在此，我们简要总结利用MALDI MS研究微生物感染致病机制的工作。
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