Liu Ming, Jin Yu-hong, Li Tiao-hong, Shi Lin-hui, Zhu Bao-qi
Ningbo Medical Treatment Center Lihuili Hospital， Ningbo 315000， China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2014 May;43(3):293-7. doi: 10.3785/j.issn.1008-9292.2014.05.006.
To investigate the effects of simvastatin on atherosclerosis and central aortic pressure in ApoE-knockout (ApoE-/-) mice.
Ten 5-week-old male ApoE-/- mice and 5 C57 mice were fed with high-lipid diet for 3 weeks, and then C57 mice (WT group) and 5 ApoE-/- mice (ApoE-/- group) were given 1% carboxymethyl cellulose solution (8 ml·kg-1·d-1), and another 5 ApoE-/- mice (ApoE-/-/S group) were given simvastatin solution (50 mg·kg-1·d-1) by gavege for 3 weeks. The areas of atherosclerotic lesion in aortic root, central aortic pressure and serum lipid levels were examined.
No atherosclerotic plaques were observed in WT group. Compared with ApoE-/- group, simvastatin significantly decreased atherosclerotic lesion area in aortic root (89 818.05±16 980.93 μm2 vs 34 937.01±13 280.65 μm2, P<0.05). The systolic pressure (SP), mean arterial pressure (MAP), pulse pressure (PP) and diastolic pressure (DP) of central aortic pressure were significantly increased in ApoE-/- group compared with those in WT group (P<0.05). Compared to ApoE-/- group, the SP, MAP and PP of central aortic pressure were significantly reduced in ApoE-/-/S group (P<0.05). SP and MAP of central aortic pressure were positively correlated with atherosclerotic lesion area (SP: r=0.7152, P=0.0461; PP: r=0.7594， P=0.0288). Compared with WT group, serum triglyceride, total cholesterol and low-density lipoprotein levels were markedly increased in ApoE-/- group (P<0.05). Serum high-density lipoprotein level was decreased in ApoE-/- group compared with WT group. No differences in serum triglyceride, total cholesterol, low-density lipoprotein and high-density lipoprotein levels were found between ApoE-/- group and ApoE-/-/S group.
Simvastatin can attenuate atherosclerosis of aorta in ApoE-/- mice, which is associated with the reduced central aortic systolic pressure but not with the serum lipids levels.
研究辛伐他汀对载脂蛋白E基因敲除（ApoE-/-）小鼠动脉粥样硬化及中心动脉压的影响。
将10只5周龄雄性ApoE-/-小鼠和5只C57小鼠给予高脂饮食3周，然后C57小鼠（野生型组）和5只ApoE-/-小鼠（ApoE-/-组）给予1%羧甲基纤维素溶液（8 ml·kg-1·d-1），另外5只ApoE-/-小鼠（ApoE-/-/辛伐他汀组）给予辛伐他汀溶液（50 mg·kg-1·d-1）灌胃3周。检测主动脉根部动脉粥样硬化病变面积、中心动脉压及血脂水平。
野生型组未观察到动脉粥样硬化斑块。与ApoE-/-组相比，辛伐他汀显著降低了主动脉根部动脉粥样硬化病变面积（89 818.05±16 980.93 μm2对34 937.01±13 280.65 μm2，P<0.05）。与野生型组相比，ApoE-/-组中心动脉压的收缩压（SP）、平均动脉压（MAP）、脉压（PP）和舒张压（DP）显著升高（P<0.05）。与ApoE-/-组相比，ApoE-/-/辛伐他汀组中心动脉压的SP、MAP和PP显著降低（P<0.05）。中心动脉压的SP和MAP与动脉粥样硬化病变面积呈正相关（SP：r=0.7152，P=0.0461；PP：r=0.7594，P=0.0288）。与野生型组相比，ApoE-/-组血清甘油三酯、总胆固醇和低密度脂蛋白水平显著升高（P<0.05）。与野生型组相比，ApoE-/-组血清高密度脂蛋白水平降低。ApoE-/-组与ApoE-/-/辛伐他汀组血清甘油三酯、总胆固醇、低密度脂蛋白和高密度脂蛋白水平无差异。
辛伐他汀可减轻ApoE-/-小鼠主动脉粥样硬化，这与中心动脉收缩压降低有关，而与血脂水平无关。
