枯草芽孢杆菌的DNA聚合酶III基因。
DNA polymerase III gene of Bacillus subtilis.
作者信息
Sanjanwala B, Ganesan A T
机构信息
Department of Genetics, Stanford Medical School, CA 94305.
出版信息
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4421-4. doi: 10.1073/pnas.86.12.4421.
Abstract
The Bacillus subtilis dnaF (polC) gene that codes for the alpha subunit of the DNA polymerase III holoenzyme has been sequenced. It consists of 4005 base pairs coding for 1335 amino acids (from the start to the stop codon), giving a molecular weight of 151,273. A mutation (azp-12) that confers resistance to the antimicrobial drug 6-(p-hydroxyphenylazo)-uracil is due to a single base change at nucleotide 3523, from TCA to GCA, resulting in a change of the 1175th amino acid, serine, to alanine. It is in the active site and located at the C-terminal part of the enzyme. The amino acid composition in an N-terminal domain has 26% homology to the epsilon subunit coded by the dnaQ gene of Escherichia coli, which is a 3'----5' proofreading exonuclease, supporting an earlier observation that this function is an integral part of the polymerase molecule in B. subtilis.
摘要
编码DNA聚合酶III全酶α亚基的枯草芽孢杆菌dnaF（polC）基因已被测序。它由4005个碱基对组成，编码1335个氨基酸（从起始密码子到终止密码子），分子量为151,273。赋予对抗菌药物6-（对羟基苯基偶氮）-尿嘧啶抗性的突变（azp-12）是由于核苷酸3523处的单个碱基变化，从TCA变为GCA，导致第1175个氨基酸丝氨酸变为丙氨酸。它位于酶的活性位点且位于C末端部分。N末端结构域中的氨基酸组成与大肠杆菌dnaQ基因编码的ε亚基具有26%的同源性，ε亚基是一种3'→5'校对核酸外切酶，这支持了早期的观察结果，即该功能是枯草芽孢杆菌中聚合酶分子的一个组成部分。
相似文献
1
DNA polymerase III gene of Bacillus subtilis.枯草芽孢杆菌的DNA聚合酶III基因。
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4421-4. doi: 10.1073/pnas.86.12.4421.
2
Bacillus subtilis DNA polymerase III: complete sequence, overexpression, and characterization of the polC gene.枯草芽孢杆菌DNA聚合酶III：polC基因的完整序列、过表达及特性分析
Gene. 1991 Feb 1;98(1):29-36. doi: 10.1016/0378-1119(91)90100-p.
3
Cloning and characterization of the polC region of Bacillus subtilis.枯草芽孢杆菌polC区域的克隆与特性分析
J Bacteriol. 1986 Mar;165(3):951-7. doi: 10.1128/jb.165.3.951-957.1986.
4
Genetic structure and domains of DNA polymerase III of Bacillus subtilis.枯草芽孢杆菌DNA聚合酶III的遗传结构和结构域
Mol Gen Genet. 1991 May;226(3):467-72. doi: 10.1007/BF00260660.
5
The cloned polC gene of Bacillus subtilis: characterization of the azp12 mutation and controlled in vitro synthesis of active DNA polymerase III.枯草芽孢杆菌的克隆polC基因：azp12突变的特征及活性DNA聚合酶III的体外可控合成
Gene. 1989 Dec 21;85(1):177-86. doi: 10.1016/0378-1119(89)90478-2.
6
Bacillus subtilis dnaF: a mutation of the gene specifying the structure of DNA polymerase III.
Mol Gen Genet. 1978 Sep 8;164(3):335-9. doi: 10.1007/BF00333165.
7
Expression of the Bacillus subtilis polC gene in Escherichia coli.枯草芽孢杆菌polC基因在大肠杆菌中的表达。
Mol Gen Genet. 1987 May;207(2-3):335-41. doi: 10.1007/BF00331598.
8
Localization of the exonuclease and polymerase domains of Bacillus subtilis DNA polymerase III.枯草芽孢杆菌DNA聚合酶III外切核酸酶结构域和聚合酶结构域的定位
Gene. 1992 Feb 1;111(1):43-9. doi: 10.1016/0378-1119(92)90601-k.
9
Leader region of the gene encoding DNA polymerase III of Bacillus subtilis.
Mol Gen Genet. 1993 Jan;236(2-3):374-8. doi: 10.1007/BF00277136.
10
Identification of the epsilon-subunit of Escherichia coli DNA polymerase III holoenzyme as the dnaQ gene product: a fidelity subunit for DNA replication.鉴定大肠杆菌DNA聚合酶III全酶的ε亚基为dnaQ基因产物：DNA复制的保真亚基。
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7085-9. doi: 10.1073/pnas.80.23.7085.
引用本文的文献
1
Bacillus subtilis DNA polymerases, PolC and DnaE, are required for both leading and lagging strand synthesis in SPP1 origin-dependent DNA replication.枯草芽孢杆菌DNA聚合酶PolC和DnaE是SPP1依赖于原点的DNA复制中前导链和后随链合成所必需的。
Nucleic Acids Res. 2017 Aug 21;45(14):8302-8313. doi: 10.1093/nar/gkx493.
2
Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family.断裂基因：一种涉及新型分裂内含肽和新型归巢内切核酸酶家族的新型基因组排列。
Nucleic Acids Res. 2009 May;37(8):2560-73. doi: 10.1093/nar/gkp095. Epub 2009 Mar 5.
3
RNase T shares conserved sequence motifs with DNA proofreading exonucleases.核糖核酸酶T与DNA校对核酸外切酶具有保守的序列基序。
Nucleic Acids Res. 1993 May 25;21(10):2521-2. doi: 10.1093/nar/21.10.2521.
4
A human retinoic acid receptor gamma isoform is homologous to the murine retinoic acid receptor gamma 7.一种人类视黄酸受体γ亚型与小鼠视黄酸受体γ7同源。
Nucleic Acids Res. 1993 May 25;21(10):2520. doi: 10.1093/nar/21.10.2520.
5
Leader region of the gene encoding DNA polymerase III of Bacillus subtilis.
Mol Gen Genet. 1993 Jan;236(2-3):374-8. doi: 10.1007/BF00277136.
6
New nucleotide sequence data on the EMBL File Server.欧洲分子生物学实验室文件服务器上的新核苷酸序列数据。
Nucleic Acids Res. 1989 Nov 11;17(21):8905-12. doi: 10.1093/nar/17.21.8905.
7
Development of novel inhibitor probes of DNA polymerase III based on dGTP analogs of the HPUra type: base, nucleoside and nucleotide derivatives of N2-(3,4-dichlorobenzyl)guanine.基于HPUra型dGTP类似物的新型DNA聚合酶III抑制剂探针的开发：N2-(3,4-二氯苄基)鸟嘌呤的碱基、核苷和核苷酸衍生物
Nucleic Acids Res. 1990 Dec 25;18(24):7381-7. doi: 10.1093/nar/18.24.7381.
8
Genetic structure and domains of DNA polymerase III of Bacillus subtilis.枯草芽孢杆菌DNA聚合酶III的遗传结构和结构域
Mol Gen Genet. 1991 May;226(3):467-72. doi: 10.1007/BF00260660.
9
Eukaryotic DNA polymerase amino acid sequence required for 3'----5' exonuclease activity.
Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9473-7. doi: 10.1073/pnas.88.21.9473.
10
Regulation of the pAD1-encoded sex pheromone response in Enterococcus faecalis: nucleotide sequence analysis of traA.粪肠球菌中pAD1编码的性信息素反应的调控：traA的核苷酸序列分析
J Bacteriol. 1992 Mar;174(6):1821-7. doi: 10.1128/jb.174.6.1821-1827.1992.
本文引用的文献
1
Mutator strains of Escherichia coli, mutD and dnaQ, with defective exonucleolytic editing by DNA polymerase III holoenzyme.大肠杆菌的突变菌株mutD和dnaQ，其DNA聚合酶III全酶的核酸外切酶编辑功能存在缺陷。
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2189-92. doi: 10.1073/pnas.80.8.2189.
2
Structure and expression of the dnaQ mutator and the RNase H genes of Escherichia coli: overlap of the promoter regions.大肠杆菌dnaQ突变基因和核糖核酸酶H基因的结构与表达：启动子区域的重叠
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7137-41. doi: 10.1073/pnas.80.23.7137.
3
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
4
A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.一种用于生成一系列连续重叠克隆以用于DNA测序的快速单链克隆策略：应用于玉米线粒体18 S rDNA测序
Plasmid. 1985 Jan;13(1):31-40. doi: 10.1016/0147-619x(85)90053-8.
5
DNA Polymerase III holoenzyme of Escherichia coli. IV. The holoenzyme is an asymmetric dimer with twin active sites.大肠杆菌的DNA聚合酶III全酶。IV. 全酶是具有双活性位点的不对称二聚体。
J Biol Chem. 1988 May 15;263(14):6570-8.
6
DNA replication.DNA复制
J Biol Chem. 1988 Jan 5;263(1):1-4.
7
Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function.噬菌体T4 DNA聚合酶诱变突变体编码的氨基酸变化。结构与功能的关系。
J Mol Biol. 1988 Aug 20;202(4):711-24. doi: 10.1016/0022-2836(88)90552-9.
8
A cryptic proofreading 3'----5' exonuclease associated with the polymerase subunit of the DNA polymerase-primase from Drosophila melanogaster.一种与黑腹果蝇DNA聚合酶-引发酶的聚合酶亚基相关的隐蔽性3'→5'核酸外切酶。
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5635-9. doi: 10.1073/pnas.84.16.5635.
9
Expression of the Bacillus subtilis polC gene in Escherichia coli.枯草芽孢杆菌polC基因在大肠杆菌中的表达。
Mol Gen Genet. 1987 May;207(2-3):335-41. doi: 10.1007/BF00331598.
10
Proofreading by DNA polymerase III of Escherichia coli depends on cooperative interaction of the polymerase and exonuclease subunits.大肠杆菌DNA聚合酶III的校对功能依赖于聚合酶和核酸外切酶亚基的协同相互作用。
Proc Natl Acad Sci U S A. 1987 Jul;84(13):4389-92. doi: 10.1073/pnas.84.13.4389.
Zotero 插件