Chung Tae-Ho, Kim Eun-Ju, Choi Ul Soo
J Zoo Wildl Med. 2014 Jun;45(2):393-6. doi: 10.1638/2013-0076R2.1.
Multiple organ invasion by keratinophilic fungi in the green iguana (Iguana iguana) has not been previously reported. In this case, a 1-yr-old female green iguana presented with a nodular, darkly discolored skin lesion surrounded by necrosis in the right ventral abdominal region. A cytologic examination of the fine needle aspiration of the lesion revealed an exuberant proliferation of fibroblasts, macrophages, and multinucleated cells along with frequent filamentous structures consistent with hyphal elements. The necropsy revealed diffuse infiltration of the liver, lung, and cardiac apex with white nodules. A histopathologic examination of the lesions also confirmed a fungal infection associated with granulomatous inflammation. Rapid polymerase chain reaction (PCR) analysis of the chitin synthase 1 gene was conducted for rapid direct detection, and inter-simple sequence repeat fingerprinting was conducted to classify the infectious origin. The PCR analysis definitively demonstrated representative Microsporum canis fungus. The present report is the first case of disseminated M. canis infection with multiorgan involvement in a green iguana.
此前尚未报道过角嗜性真菌在绿鬣蜥（Iguana iguana）体内的多器官侵袭情况。在此病例中，一只1岁雌性绿鬣蜥右侧腹侧腹部区域出现一个结节状、颜色深暗的皮肤病变，周围伴有坏死。对该病变进行细针穿刺抽吸的细胞学检查显示，成纤维细胞、巨噬细胞和多核细胞大量增殖，同时频繁出现与菌丝成分一致的丝状结构。尸检发现肝脏、肺和心尖有白色结节弥漫性浸润。对病变进行组织病理学检查也证实了与肉芽肿性炎症相关的真菌感染。进行了几丁质合酶1基因的快速聚合酶链反应（PCR）分析以进行快速直接检测，并进行了简单序列重复区间指纹分析以对感染源进行分类。PCR分析明确证实为代表性的犬小孢子菌。本报告是首例绿鬣蜥发生犬小孢子菌播散性感染并累及多个器官的病例。
