Kumar Jitendra, Kumar Jitesh, Singh Sudhir P, Tuli Rakesh
Department of Biotechnology, National Agri-Food Biotechnology Institute, Mohali, 160071, Punjab, India.
Arch Virol. 2014 Nov;159(11):3071-6. doi: 10.1007/s00705-014-2149-5. Epub 2014 Jul 8.
βC1 proteins, encoded by betasatellites, are known to be pathogenicity determinants, and they are responsible for symptom expression in many devastating diseases caused by begomoviruses. We report the involvement of βC1 in pathogenicity determination of a mastrevirus. Analysis of field samples of wheat plants containing wheat dwarf India virus (WDIV) revealed the presence of a full-length and several defective betasatellite molecules. The detected betasatellite was identified as ageratum yellow leaf curl betasatellite (AYLCB). No begomovirus was detected in any of the samples. The full-length AYLCB contained an intact βC1 gene, whereas the defective molecules contained complete or partial deletions of βC1. Agroinoculation of wheat with the full-length AYLCB and WDIV or of tobacco with ageratum enation virus enhanced the pathogenicity and accumulation of the respective viruses, whereas the defective molecules could not. This study indicates that βC1 is a pathogenicity determinant for WDIV and can interact functionally not only with begomoviruses but also with a mastrevirus.
由卫星病毒编码的βC1蛋白是已知的致病性决定因子，它们在许多由双生病毒引起的毁灭性疾病中负责症状表达。我们报道了βC1参与一种玉米线条病毒的致病性决定。对含有印度小麦矮缩病毒（WDIV）的小麦植株田间样本的分析揭示了全长和几个缺陷型卫星分子的存在。检测到的卫星分子被鉴定为藿香黄脉曲叶卫星分子（AYLCB）。在任何样本中均未检测到双生病毒。全长AYLCB包含一个完整的βC1基因，而缺陷型分子则包含βC1的完全或部分缺失。用全长AYLCB和WDIV对小麦进行农杆菌接种，或用藿香耳突病毒对烟草进行农杆菌接种，可增强相应病毒的致病性和积累，而缺陷型分子则不能。本研究表明，βC1是WDIV的致病性决定因子，不仅能在功能上与双生病毒相互作用，还能与玉米线条病毒相互作用。
