[Foxp3、T-bet和GATA-3表达失衡与慢性阻塞性肺疾病炎症相关]

[Imbalance in the expression of Foxp3, T-bet and GATA-3 is correlated with inflammation of chronic obstructive pulmonary disease].

作者信息

Wang Chengyang, Liu Xiangguo, Peng Qinghe, Li Da, Ji Qiaoxue, Wang Chuanbo, Li Zegeng

机构信息

Graduate School, Hubei University of Traditional Chinese Medicine, Wuhan 430065; Clinical College of Integrated Chinese and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.

Clinical College of Integrated Chinese and Western Medicine, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Jul;30(7):704-7, 712.

PMID:25001933

Abstract

OBJECTIVE

To observe the change of regulatory T cells(Tregs), fork head-like transcription factor 3 (Foxp3), T box expressed in T cells (T-bet) and GATA binding protein 3 (GATA3) in rat models of chronic obstructive pulmonary disease (COPD).

METHODS

Thirty rats were randomly divided into control group and model group (n=15 each). The rats of model group were developed by lipopolysaccharide (LPS) and smoking. Interleukin-4 (IL-4) and γ-interferon (IFN-γ) in serum and bronchoalveolar lavage fluid (BALF) were detected 28 days after modeling by ELISA. Peripheral Tregs were detected by flow cytometry. The expressions of Foxp3, T-bet, GATA gene and protein in lung tissue were observed by reverse transcription PCR and Western blotting, respectively.

RESULTS

Compared with the control group, the model group had more serious inflammation in lung tissues, expressed the higher levels of IFN-γ in serum and BALF (P<0.01), T-bet mRNA and protein in lung tissue, and the lower levels of IL-4, CD4⁺ CD25⁺ Treg, CD4⁺ CD25⁺ Foxp3⁺ Treg (P<0.05), Foxp3, GATA-3 mRNA and protein (P<0.01). Correlation analysis showed that there were correlations between T-bet, GATA-3, Foxp3 expressions and IL-4, IFN-γ levels (P<0.05).

CONCLUSION

There is a relationship between inflammation and imbalance of in the expression of T-bet, GATA-3, Foxp3 in COPD.

摘要

目的

观察慢性阻塞性肺疾病（COPD）大鼠模型中调节性T细胞（Tregs）、叉头样转录因子3（Foxp3）、T细胞特异性转录因子（T-bet）和GATA结合蛋白3（GATA3）的变化。

方法

将30只大鼠随机分为对照组和模型组，每组15只。模型组大鼠采用脂多糖（LPS）联合烟熏法造模。造模28天后，采用酶联免疫吸附测定（ELISA）法检测血清及支气管肺泡灌洗液（BALF）中白细胞介素-4（IL-4）和γ-干扰素（IFN-γ）水平。采用流式细胞术检测外周血Tregs。分别采用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法观察肺组织中Foxp3、T-bet、GATA基因及蛋白表达。

结果

与对照组比较，模型组肺组织炎症更严重，血清及BALF中IFN-γ水平、肺组织中T-bet mRNA及蛋白表达升高（P<0.01），IL-4、CD4⁺CD25⁺Treg、CD4⁺CD25⁺Foxp3⁺Treg水平、Foxp3、GATA-3 mRNA及蛋白表达降低（P<0.05或P<0.01）。相关性分析显示，T-bet、GATA-3、Foxp3表达与IL-4、IFN-γ水平相关（P<0.05）。