Xia Rui, Wang Feng, Gao Tengfei, Wen Wen, Lu Binfeng, Zhu Yibei, Zhang Xueguang
School of Biology & Basic Medical Sciences, Soochow University, Suzhou 215007, China.
Jiangsu Institute of Clinical Immunology, First Affiliated Hospital, Soochow University, Suzhou 215006, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Jul;30(7):740-3.
To investigate the number of myeloid-derived suppressor cells (MDSCs) in peripheral blood, tumor tissue and para-tumor normal tissues in patients with gastric cancer in an attempt to explore the relationship between MDSCs expression and clinicopathologic characteristics.
Peripheral blood was collected from 62 gastric cancer patients and 20 healthy volunteers (HC group). Gastric cancer tissues and adjacent normal tissues were obtained from 12 of the 62 gastric cancer patients. HLA-DR⁻ CD33⁺ CD11b⁺ MDSCs were analyzed by flow cytometry. Student's t-test, One-way ANOVA and Mann-Whitney U test were used to explore the correlation between MDSCs expression in peripheral blood and the depth of tumor invasion, degree of differentiation, TNM stage and lymph node metastasis.
Compare with the HC group, the number of MDSCs in peripheral blood of newly-diagnosed gastric cancer patients was higher (P<0.01). The number of MDSCs in peripheral blood of gastric cancer patients was significantly associated with the depth of invasion, degree of differentiation, TNM stage and lymph node metastasis (P<0.05). The expression of MDSCs from gastric cancer tissues was obviously higher than that of the adjacent tissues in the same patient. The number of MDSCs in peripheral blood from recurrent/metastasis group was obviously higher than that from non-recurrent/metastasis group (P<0.05). In addition, compare with the HC group, the expression of negative costimulatory molecule T-cell immunoglobulin- and mucin-domain-containing molecule 3 (Tim-3) on HLA-DR⁻ CD33⁺ CD11b⁺ MDSCs in peripheral blood was higher in patients with gastric cancer.
MDSCs expression in peripheral blood of gastric cancer patients was closely associated with tumor malignant degree and tumor recurrence/metastasis.
研究胃癌患者外周血、肿瘤组织及癌旁正常组织中髓源性抑制细胞（MDSCs）的数量，以探讨MDSCs表达与临床病理特征之间的关系。
收集62例胃癌患者及20名健康志愿者（HC组）的外周血。从62例胃癌患者中的12例获取胃癌组织及癌旁正常组织。采用流式细胞术分析HLA-DR⁻CD33⁺CD11b⁺ MDSCs。采用Student's t检验、单因素方差分析和Mann-Whitney U检验探讨外周血中MDSCs表达与肿瘤浸润深度、分化程度、TNM分期及淋巴结转移之间的相关性。
与HC组相比，新诊断胃癌患者外周血中MDSCs数量更高（P<0.01）。胃癌患者外周血中MDSCs数量与浸润深度、分化程度、TNM分期及淋巴结转移显著相关（P<0.05）。同一患者胃癌组织中MDSCs的表达明显高于癌旁组织。复发/转移组外周血中MDSCs数量明显高于非复发/转移组（P<0.05）。此外，与HC组相比，胃癌患者外周血中HLA-DR⁻CD33⁺CD11b⁺ MDSCs上负性共刺激分子T细胞免疫球蛋白黏蛋白结构域分子3（Tim-3）的表达更高。
胃癌患者外周血中MDSCs表达与肿瘤恶性程度及肿瘤复发/转移密切相关。
