Shalaby M R, Waage A, Espevik T
Institute of Cancer Research, University of Trondheim, Norway.
Cell Immunol. 1989 Jul;121(2):372-82. doi: 10.1016/0008-8749(89)90036-1.
The influence of recombinant (r) human tumor necrosis factor alpha (rTNF-alpha), r human interleukin 1 beta (rIL-1 beta), and r human interferon gamma (rIFN-gamma) on the production of interleukin 6 (IL-6) by human endothelial cells (HEC) was investigated. The addition of 1-100 U/ml of either rTNF-alpha or rIL-1 beta to cultures of HEC monolayers caused a dose-related increase in IL-6 production as detected after 24 hr of incubation. In contrast to rIL-1 beta and rTNF-alpha, the use of up to 1000 U/ml of rIFN-gamma caused only a moderate increase in IL-6 production. However, significantly greater quantities of IL-6 were produced by HEC monolayers subjected to 1000 U/ml of rIFN-gamma in combination with 1-100 U/ml of rTNF-alpha. Furthermore, the addition of graded concentrations of human transforming growth factor beta (TGF-beta) to cultures resulted in a dose-related inhibition of rIL-1 beta- and rTNF-alpha-induced IL-6 production by HEC. The results demonstrate that rIL-1 beta and rTNF-alpha share the ability to stimulate HEC for production of IL-6 and indicate that TGF-beta may act as an immunosuppressive agent, at least partially, through its ability to inhibit the action of TNF-alpha and IL-1 on endothelial cells.
研究了重组（r）人肿瘤坏死因子α（rTNF-α）、r人白细胞介素1β（rIL-1β）和r人干扰素γ（rIFN-γ）对人内皮细胞（HEC）白细胞介素6（IL-6）产生的影响。向HEC单层培养物中添加1-100 U/ml的rTNF-α或rIL-1β，孵育24小时后检测到IL-6产生呈剂量相关增加。与rIL-1β和rTNF-α不同，使用高达1000 U/ml的rIFN-γ仅导致IL-6产生适度增加。然而，与1-100 U/ml的rTNF-α联合使用1000 U/ml的rIFN-γ时，HEC单层产生的IL-6量显著增加。此外，向培养物中添加分级浓度的人转化生长因子β（TGF-β）导致HEC对rIL-1β和rTNF-α诱导的IL-6产生呈剂量相关抑制。结果表明，rIL-1β和rTNF-α具有共同刺激HEC产生IL-6的能力，并表明TGF-β可能至少部分地通过其抑制TNF-α和IL-1对内皮细胞作用的能力而作为一种免疫抑制剂发挥作用。
