Campanholo Vanessa Maria de Lima Pazine, Felipe Aledson Vitor, de Lima Jacqueline Miranda, Pimenta Célia Aparecida Marques, Ventura Rogéria Maria, Forones Nora Manoukian
Grupo de Oncologia, Disciplina de Gastroenterologia, Universidade Federal de São Paulo, Brasil, São Paulo, SP, Brasil.
Arq Gastroenterol. 2014 Apr-Jun;51(2):79-83. doi: 10.1590/s0004-28032014000200002.
Genomic alterations play important roles in gastric cancer carcinogenesis. Cyclooxygenases (COX) are important enzymes in the maintenance of mucosal integrity and in pathological processes, mainly in inflammation and cancer. The -765G>C COX-2 polymorphism has been implicated in gastric cancer risk.
To evaluate the COX-2 gene polymorphism as a predictor of gastric cancer risk.
One hundred gastric cancer patients and 150 controls were enrolled from a Brazilian centre. Personal data regarding related risk factors, including alcohol consumption and smoking behavior, were collected via questionnaire. DNA was extracted from peripheral blood and the genotypes were analyzed using PCR-restriction fragment length polymorphism.
G/G, G/C and C/C genotypes frequencies was 42.7%, 50% and 7.3%, respectively in controls and 59.0%, 34.0% and 7.0% in gastric cancer. The frequency of the genotypes differed between the groups (P = 0.033). A higher risk of gastric cancer was associated with COX-2 -765G/G genotype (P = 0.048; OR:1.98, 95% CI = 1.01-3.90). Alcohol consumption and smoking in patients with -765G/G genotype also increased the risk of gastric cancer.
The -765G/G genotype and the -765G allele had been associated with an increased risk for gastric cancer. The presence of smoking and alcohol consumption increased the risk for gastric cancer in subjects with -765G/G genotype compared with the control group. Polymorphism of COX-2 gene and gastric cancer risk.
基因组改变在胃癌发生过程中起重要作用。环氧化酶（COX）是维持黏膜完整性及参与病理过程（主要是炎症和癌症）的重要酶。COX -2基因-765G>C多态性与胃癌风险有关。
评估COX -2基因多态性作为胃癌风险预测指标的价值。
从巴西一个中心招募了100例胃癌患者和150例对照。通过问卷调查收集包括饮酒和吸烟行为等相关危险因素的个人数据。从外周血中提取DNA，并使用聚合酶链反应-限制性片段长度多态性分析基因型。
对照组中G/G、G/C和C/C基因型频率分别为42.7%、50%和7.3%，胃癌组中分别为59.0%、34.0%和7.0%。两组间基因型频率存在差异（P = 0.033）。COX -2 -765G/G基因型与胃癌风险增加相关（P = 0.048；比值比：1.98，95%置信区间 = 1.01 - 3.90）。-765G/G基因型患者饮酒和吸烟也增加了胃癌风险。
-765G/G基因型和-765G等位基因与胃癌风险增加有关。与对照组相比，-765G/G基因型个体中吸烟和饮酒的存在增加了胃癌风险。COX -2基因多态性与胃癌风险。
