Guo W L, Zhang Q, Wang J
Department of Radiology, The Children's Hospital Affiliated to Soochow University, Suzhou, China.
Department of General Surgery, The Children's Hospital Affiliated to Soochow University, Suzhou, China.
Braz J Med Biol Res. 2014 Jul;47(7):594-9. doi: 10.1590/1414-431x20142765. Epub 2014 May 30.
β-catenin and c-myc play important roles in the development of tissues and organs. However, little is known about their expression patterns during the development of the human common bile duct. Immunohistochemistry was used to detect β-catenin and c-myc expression in common bile duct samples from postmortem tissues of 14 premature infants and 6 spontaneously aborted fetuses. The expression of β-catenin and c-myc was also analyzed by Western blot. The samples were divided into four groups based on the stage of human fetal development: 12, 13-27, 28-37, and >37 weeks. The Image-Pro Plus v. 6.0 image analysis software was used to calculate the mean qualifying score (MQS). At fetal stages 12, 13-27, 28-37, and >37 weeks, MQS of β-catenin were 612.52 ± 262.13, 818.38 ± 311.73, 706.33 ± 157.19, and 350.69 ± 110.19, respectively. There was a significant difference in MQS among the four groups (ANOVA, P=0.0155) and between the scores at >37 and 13-27 weeks (Student-Newman-Keuls, P<0.05). At fetal stages 12, 13-27, 28-37, and >37 weeks, the MQS of c-myc were 1376.64 ± 330.04, 1224.18 ± 171.66, 1270.24 ± 320.75, and 741.04 ± 219.19, respectively. There was a significant difference in MQS among the four groups (ANOVA, P=0.0087) and between the scores at >37 and 12 weeks, >37 and 13-27 weeks, and >37 and 28-37 weeks (all P<0.05, Student-Newman-Keuls). Western blots showed that β-catenin and c-myc expression were significantly higher in fetal than in postnatal control duct tissue (P<0.05). c-myc and β-catenin are involved in the normal development of the human common bile duct.
β-连环蛋白和c-myc在组织和器官发育中发挥重要作用。然而，关于它们在人类胆总管发育过程中的表达模式却知之甚少。采用免疫组织化学法检测14例早产婴儿和6例自然流产胎儿尸体解剖组织胆总管样本中β-连环蛋白和c-myc的表达。同时通过蛋白质印迹法分析β-连环蛋白和c-myc的表达。根据人类胎儿发育阶段将样本分为四组：12周、13 - 27周、28 - 37周和>37周。使用Image-Pro Plus v. 6.0图像分析软件计算平均合格分数（MQS）。在胎儿12周、13 - 27周、28 - 37周和>37周时，β-连环蛋白的MQS分别为612.52±262.13、818.38±311.73、706.33±157.19和350.69±110.19。四组间MQS存在显著差异（方差分析，P = 0.0155），且>37周与13 - 27周的分数之间存在显著差异（Student-Newman-Keuls检验，P<0.05）。在胎儿12周、13 - 27周、28 - 37周和>37周时，c-myc的MQS分别为1376.64±330.04、1224.18±171.66、1270.24±320.75和741.04±219.19。四组间MQS存在显著差异（方差分析，P = 0.0087），且>37周与12周、>37周与13 - 27周以及>37周与28 - 37周的分数之间均存在显著差异（均为P<0.05，Student-Newman-Keuls检验）。蛋白质印迹显示，胎儿胆总管组织中β-连环蛋白和c-myc的表达明显高于出生后对照胆管组织（P<0.05）。c-myc和β-连环蛋白参与人类胆总管的正常发育。
