Huang Hai, Wei Yun, Meng Zining, Zhang Yong, Liu Xiaochun, Guo Liang, Luo Jian, Chen Guohua, Lin Haoran
Sanya Science & Technology Academy of Hainan National Breeding and Multiplication, Sanya 572000, China.
Key Laboratory of Tropical Biology Resources, Ministry of Education, College of Ocean, Hainan University, Haikou 570228, China.
Int J Mol Sci. 2014 Jul 7;15(7):11996-2006. doi: 10.3390/ijms150711996.
In mammals, leptin has been demonstrated to perform important roles in many physiological activities and to influence development, growth, metabolism and reproduction. However, in fish, its function is still unclear. Duplicate leptin genes, leptin-a and leptin-b, have been identified in the orange-spotted grouper. In the present study, the polymorphisms in the leptin-b gene of the orange-spotted grouper were detected, and the relation between these polymorphisms and 12 growth traits were analyzed. Six polymorphisms (including 3 single nucleotide polymorphisms (c.14G>A, c.93A>G, c.149G>A) in exon 1, 2 SNPs (c.181A>G, c.193G>A) in intron 1, and 1 SNP (c.360C>T) in exon 2) were identified and genotyped from 200 different individuals. The results revealed that the SNP c.149G>A was significantly associated with growth traits, that the heterozygous mutation genotype GA having negative effects on growth traits. However, the other five SNPs (c.14G>A, c.93A>G, c.181A>G, c.193G>A, c.360C>T) did not show significant associations with all the growth traits. Compared with our findings in leptin-a gene, the results suggested that the leptin-a hormone has more important physiological effects in fish bodies than the leptin-b type. Moreover, leptin genes were supposed to be one class of major candidate genes of regulating growth traits in the orange-spotted grouper.
在哺乳动物中，瘦素已被证明在许多生理活动中发挥重要作用，并影响发育、生长、代谢和繁殖。然而，在鱼类中，其功能仍不清楚。在点带石斑鱼中已鉴定出重复的瘦素基因，即瘦素-a和瘦素-b。在本研究中，检测了点带石斑鱼瘦素-b基因的多态性，并分析了这些多态性与12个生长性状之间的关系。从200个不同个体中鉴定并基因分型了6个多态性（包括外显子1中的3个单核苷酸多态性（c.14G>A、c.93A>G、c.149G>A）、内含子1中的2个单核苷酸多态性（c.181A>G、c.193G>A）和外显子2中的1个单核苷酸多态性（c.360C>T））。结果显示，单核苷酸多态性c.149G>A与生长性状显著相关，杂合突变基因型GA对生长性状有负面影响。然而，其他5个单核苷酸多态性（c.14G>A、c.93A>G、c.181A>G、c.193G>A、c.360C>T）与所有生长性状均无显著关联。与我们在瘦素-a基因中的发现相比，结果表明瘦素-a激素在鱼体中的生理作用比瘦素-b类型更重要。此外，瘦素基因被认为是点带石斑鱼生长性状调控的一类主要候选基因。
