Keevil C W, Spillane B J
Pathogenic Microbes Research Laboratory, PHLS Centre for Applied Microbiology and Research, Salisbury, U.K.
FEMS Microbiol Lett. 1989 May;50(1-2):83-6. doi: 10.1111/j.1574-6968.1989.tb03087.x.
Neisseria gonorrhoeae strain P9-2 was grown in iron-limited or replete continuous culture at a dilution rate of 0.05 h-1, in the presence and absence of Staphylococcus epidermidis. Gonococci maintained expression of pili (P+) and the transparent colony phenotype in pure culture during transitions of iron- and cystine-limited growth. They competed well with staphylococci during iron-limited co-culture and comprised greater than 95% of the population. Transition to cystine-limited growth allowed the staphylococcus to predominate but the gonococcus did not wash out. Furthermore, the gonococcal opaque colony phenotype (O+), indicating synthesis of outer membrane proteins II, was now expressed. Restoration of iron limitation returned the co-culture to its original composition but with P+O+ gonococci dominating. These results suggest that environments might exist in Man where gonococci can compete successfully with normal indigenous bacteria during infection.
淋病奈瑟菌菌株P9 - 2在铁限制或充足的连续培养中以0.05 h⁻¹的稀释率生长，培养环境中存在或不存在表皮葡萄球菌。在铁和胱氨酸限制生长的转变过程中，淋球菌在纯培养中保持菌毛表达（P⁺）和透明菌落表型。在铁限制的共培养中，它们与葡萄球菌竞争良好，占菌群的95%以上。向胱氨酸限制生长的转变使葡萄球菌占主导，但淋球菌并未被淘汰。此外，此时表达了表明外膜蛋白II合成的淋球菌不透明菌落表型（O⁺）。铁限制的恢复使共培养恢复到原来的组成，但此时P⁺O⁺淋球菌占主导。这些结果表明，在人体中可能存在这样的环境，即淋球菌在感染期间能够与正常的本土细菌成功竞争。
