毒蕈碱激动剂毛果芸香碱抑制外周血单核细胞中的DNA和γ干扰素合成。

The muscarinic agonist pilocarpine inhibits DNA and interferon-gamma synthesis in peripheral blood mononuclear cells.

作者信息

Arzt E S, Fernández-Castelo S, Diaz A, Finkielman S, Nahmod V E

机构信息

Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), República Argentina.

出版信息

Int J Immunopharmacol. 1989;11(3):275-81. doi: 10.1016/0192-0561(89)90165-3.

DOI:10.1016/0192-0561(89)90165-3

PMID:2500403

Abstract

Lymphocyte basal DNA synthesis and proliferative responses to phytohemagglutinin (PHA) showed a dose-dependent (5 X 10(-5)-5 X 10(-3) M) inhibition by the muscarinic agonist pilocarpine, in contrast to the basal enhancing effect produced by the M2 muscarinic-nicotinic agonist carbachol. The effect of pilocarpine was reversed by both atropine (1 X 10(-6) M) and pirenzepine (1 X 10(-7)-1 X 10(-8) M), M1-M2 and M1 muscarinic antagonists, respectively. The effect of pilocarpine may thus be specific for the M1 muscarinic receptor. Pilocarpine also inhibited interferon-gamma (IFN-gamma)-PHA induced production, but was unable to reverse the pokeweed mitogen (PWM)-induced DNA synthesis. Distinct immunoregulatory activities are suggested for cholinergic muscarinic receptors M1 and M2.

摘要

与M2毒蕈碱 - 烟碱激动剂卡巴胆碱产生的基础增强作用相反，毒蕈碱激动剂毛果芸香碱对淋巴细胞基础DNA合成及对植物血凝素（PHA）的增殖反应呈现剂量依赖性（5×10⁻⁵ - 5×10⁻³ M）抑制作用。毛果芸香碱的作用分别被M1 - M2和M1毒蕈碱拮抗剂阿托品（1×10⁻⁶ M）和哌仑西平（1×10⁻⁷ - 1×10⁻⁸ M）逆转。因此，毛果芸香碱的作用可能对M1毒蕈碱受体具有特异性。毛果芸香碱还抑制γ - 干扰素（IFN - γ） - PHA诱导的产生，但无法逆转商陆丝裂原（PWM）诱导的DNA合成。提示胆碱能毒蕈碱受体M1和M2具有不同的免疫调节活性。