铜绿假单胞菌IID 1009（ATCC 27585）O-多糖的结构

The structure of the O-polysaccharide from Pseudomonas aeruginosa IID 1009 (ATCC 27585).

作者信息

Kaya S, Araki Y, Ito E

机构信息

Department of Chemistry, Faculty of Science, Hokkaido University.

出版信息

J Biochem. 1989 Jan;105(1):35-8. doi: 10.1093/oxfordjournals.jbchem.a122613.

DOI:10.1093/oxfordjournals.jbchem.a122613

PMID:2500429

Abstract

Structural studies were carried out on the O-polysaccharide fraction obtained by mild acid treatment of the lipopolysaccharide from Pseudomonas aeruginosa IID 1009 (ATCC 27585). The O-polysaccharide was composed of L-rhamnose, N-acetyl-D-quinovosamine, and N-acetyl-L-galactosaminuronic acid in a molar ratio of 1:1:1. The results from analysis of fragments obtained by hydrogen fluoride hydrolysis of O-polysaccharide, together with data on methylation analysis and nuclear magnetic resonance spectroscopic analysis, led to the most likely structure of the repeating units of the polymer chain ----4)L-GalNAcA(alpha 1----3)D-QuiNAc(alpha 1----3)L-Rha(alpha 1----, in which about 70% of the rhamnose residues were O-acetylated at C-2. This structure coincides with that of the repeating unit of Lanyi 02 a,b polysaccharides.

摘要

对通过温和酸处理铜绿假单胞菌IID 1009（ATCC 27585）脂多糖获得的O-多糖部分进行了结构研究。该O-多糖由L-鼠李糖、N-乙酰-D-奎诺糖胺和N-乙酰-L-半乳糖胺糖醛酸以1:1:1的摩尔比组成。通过对O-多糖进行氟化氢水解得到的片段分析结果，以及甲基化分析和核磁共振光谱分析数据，得出了聚合物链重复单元的最可能结构----4)L-GalNAcA(α1----3)D-QuiNAc(α1----3)L-Rha(α1----，其中约70%的鼠李糖残基在C-2位被O-乙酰化。该结构与Lanyi 02 a,b多糖重复单元的结构一致。